The Effects of GM-CSF, HB-EGF and LIF Cytokines Supplementation Need Verification for Human Embryo Development in Vitro
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 443
- 试验地点
- 2
- 主要终点
- ongoing pregnancy rate ( % )
研究概览
简要总结
Assessment of the embryological benefit of in vitro adding of Cytokines (LIF 5ng/ml, HB-EGF 5ng/ml and GM-CSF 2n/ml) to the culture media and closely monitor its effect on embryogenesis and implantation outcomes.
详细描述
The IVF culture media design and upgrade underwent many ideas during the last 3 decades, we suppose that adding the Cytokines (LIF 5ng/ml, HB-EGF 5ng/ml and GM-CSF 2ng/ml) as a growth factor that have multi-ways to might improve embryo development in vitro so we decide to design a trial to compare the embryogenesis by sibling Oocytes of the same patient between two arm of the same culture media and the same culture condition. One arm with Cytokines and the other is traditional one and closely monitor the outcome within the 2 arms and record the results.
研究设计
- 研究类型
- Interventional
- 分配方式
- Randomized
- 干预模型
- Parallel
- 主要目的
- Other
- 盲法
- Triple (Participant, Care Provider, Outcomes Assessor)
入排标准
- 年龄范围
- 18 Years 至 40 Years（Adult）
- 性别
- Female
- 接受健康志愿者
- 是
入选标准
- •all patients
排除标准
- •over 40 years
研究组 & 干预措施
Cytokines supplemented In Vitro single culture media
We will add Cytokines (LIF, HB-EGF and GM-CSF) to LIFEGLOBAL culture media and assess the embryogenesis and pregnancy.
干预措施: Adding LIF, HB-EGF and GM-CSF to Lifeglobal human IVF culture media (Other)
Traditional Single step culture media
结局指标
主要结局
ongoing pregnancy rate ( % )
时间窗: 6 months
次要结局
- top-quality embryo rate(6 days of culture)
- rates for blastocyst formation and quality(6 days of culture)
- clinical pregnancy rate ( % )(6 months)
- implantation rate(6 months)
- embryo utilization rate(6 days of culture)
- fertilization rate(6 days of culture)
研究者
Muhammad Fawzy
IVF Laboratory Director
Ibn Sina Hospital