Adding Cytokines to In Vitro Human Culture Media to Improve Embryogenesis and Implantation
- Conditions
- Infertility
- Registration Number
- NCT02420886
- Lead Sponsor
- Ibn Sina Hospital
- Brief Summary
Assessment of the embryological benefit of in vitro adding of Cytokines (LIF 5ng/ml, HB-EGF 5ng/ml and GM-CSF 2n/ml) to the culture media and closely monitor its effect on embryogenesis and implantation outcomes.
- Detailed Description
The IVF culture media design and upgrade underwent many ideas during the last 3 decades, we suppose that adding the Cytokines (LIF 5ng/ml, HB-EGF 5ng/ml and GM-CSF 2ng/ml) as a growth factor that have multi-ways to might improve embryo development in vitro so we decide to design a trial to compare the embryogenesis by sibling Oocytes of the same patient between two arm of the same culture media and the same culture condition. One arm with Cytokines and the other is traditional one and closely monitor the outcome within the 2 arms and record the results.
Recruitment & Eligibility
- Status
- COMPLETED
- Sex
- Female
- Target Recruitment
- 443
- all patients
- over 40 years
Study & Design
- Study Type
- INTERVENTIONAL
- Study Design
- PARALLEL
- Primary Outcome Measures
Name Time Method ongoing pregnancy rate ( % ) 6 months
- Secondary Outcome Measures
Name Time Method top-quality embryo rate 6 days of culture rates for blastocyst formation and quality 6 days of culture clinical pregnancy rate ( % ) 6 months fertilization rate 6 days of culture embryo utilization rate 6 days of culture implantation rate 6 months
Trial Locations
- Locations (2)
Banon IVF Center
🇪🇬Assiut, Egypt
Ibnsina IVF center
🇪🇬Sohag, Egypt
Banon IVF Center🇪🇬Assiut, Egypt