Expression of miRNa-138 and Cyclin D1 in Oral Mucosa of Patients With Oral Lichen Planus
- Conditions
- Oral Lichen Planus
- Registration Number
- NCT02834520
- Lead Sponsor
- Cairo University
- Brief Summary
60 individuals were subdivided into 2 groups, 30 patients with oral lichen planus, 30 control individuals. Expression of miRNA-138 and cyclin D1 were evaluated in oral mucosa utilizing Quantitative real-time polymerase chain reaction and immunohistochemistry.
- Detailed Description
60 individuals were subdivided into 3 groups, into 2 groups, 30 patients with oral lichen planus, 30 control individuals having age, gender and periodontal status matched with both patients groups acting as a control group. Relative gene expression of miRNA-138 was estimated using Quantitative real-time polymerase chain reaction technique in oral mucosa of both oral lichen planus patients and control subjects. Sub grouping of oral lichen planus patients were performed into reticular, atrophic and erosive groups (n=10 each). Cyclin D1 gene expression was estimated using Quantitative real-time polymerase chain reaction technique and Cyclin D1 protein level was estimated using immunohistochemistry.
Recruitment & Eligibility
- Status
- COMPLETED
- Sex
- All
- Target Recruitment
- 60
- patients with oral lichen planus.
- any systemic disease other than oral lichen planus.
- smokers.
- pregnant females.
- periodontal diseases.
Study & Design
- Study Type
- OBSERVATIONAL
- Study Design
- Not specified
- Primary Outcome Measures
Name Time Method expression of miRNA-138 1 day expression of miRNA-138 in oral mucosa of patients with oral lichen planus
- Secondary Outcome Measures
Name Time Method gene expression of cyclin D1 1 day gene expression of cyclin D1 in oral mucosa of patients with oral lichen planus
protein expression of cyclin D1 1 day protein expression of cyclin D1 in oral mucosa of patients with oral lichen planus
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