Understanding the Determinants of Mucosal Immunity and Optimizing the Diagnosis of Infection With SARS-CoV-2 Variants
试验速览
- 阶段
- 不适用
- 状态
- 招募中
- 入组人数
- 90
- 试验地点
- 2
- 主要终点
- To characterize SARS-CoV-2 infection and host response, particularly mucosal immunity.
研究概览
简要总结
One of the current health challenges in the face of the COVID-19 pandemic that started in Wuhan in 2019, and still responsible for successive waves, is to better understand and diagnose the infection.
The new variants - delta, then omicron, which appeared in November 2021 and then their sub-variants BA.2, then BA.4 and 5, and more recently BQ.1 and the sub-variant XBB.1.5 are increasingly transmissible and responsible for some degree of immune escape. Hence the importance of a better understanding of infection- or vaccine-induced immunity in order to optimize existing prophylactic or therapeutic strategies, or even to develop new, more effective ones.
Mucosal immunity could play a particularly important role in interrupting the infection cycle at the entry point of the virus.
The key role of innate immunity has been demonstrated in particular, via interferons and the composition of the microbiota.
Humoral immunity is the best documented. However, it tends to be eroded within a few months. On the other hand, cellular immunity is more stable over time and would largely explain the decrease in severe forms of the disease in vaccinated individuals.
The collection of biological resources that will be built up during this study will also allow us to optimize or develop new diagnostic methods, necessary as a complement to vaccination, to effectively slow down the spread of the pandemic and reduce the severity of its impact on the population.
The improvement of diagnostic methods will in turn improve the understanding of the infection by providing increasingly reliable information on the characteristics of an infection, its quantification, its dynamics, and its resolution, especially since these parameters will be compared, at any time during the study, with reference methods and the immunological status of the subject.
The main significant improvements expected in the field of SARS-CoV-2 diagnosis are notably the improvement of performance (reduction of false negatives in RT-PCR on nasopharyngeal samples), acceptability, simplicity of implementation in the field, and the capacity to test transmission.
The objective of this study is to identify and characterize SARS-CoV-2 infection and host response, particularly mucosal immunity.
详细描述
This is a prospective, longitudinal, descriptive study that will include adult participants infected and uninfected with Sars-CoV-2 at the time of recruitment.
Participants will be divided into 3 groups of 30 evaluable subjects:
- uninfected,
- asymptomatically infected,
- symptomatically infected.
The participants will be identified within the Ile-de-France medical analysis laboratories partners of the project.
Study with sample collection:
研究设计
- 研究类型
- Interventional
- 分配方式
- Na
- 干预模型
- Single Group
- 主要目的
- Basic Science
- 盲法
- None
入排标准
- 年龄范围
- 18 Years 至 65 Years（Adult, Older Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •Common criteria for all subjects:
- •Aged between 18 and 65 years included
- •Whose weight is greater than or equal to 50 kg and whose state of health is compatible with the collection of 55 ml of blood at one time and 111 ml in 28 days
- •Residing in the Ile-de-France region and able to travel to the 15th arrondissement of Paris for visits to ICAReB-Clin
- •Having given their consent to participate in the study
- •Benefiting from a Social Security scheme except for the Aide Médicale d'Etat
- •Criteria for the SARS-CoV-2 infected participant group:
- •Subject tested positive for SARS-CoV-2 by RT-PCR in one of the participating laboratories for less than 72 hours
- •Asymptomatic or with symptoms not requiring hospitalization regardless of previous vaccination or infection status for SARS-CoV-
- •Criteria for the SARS-CoV-2 uninfected group:
- •Having tested negative for SARS-CoV-2 by RT-PCR
- •Subject with no more than 3 co-morbidities listed by the HAS.
排除标准
- •Criteria common to all subjects :
- •Subject under a protective measure (e.g., guardianship)
- •Participant in another biomedical research
- •For women: pregnant or breastfeeding women (declarative)
- •Subject with another acute infectious disease
- •SARS-CoV-2 RT-PCR result older than 3 days
- •Existence of at least 3 co-morbidities known to be factors of severity (and therefore representing risks of hospitalisation during follow-up)
- •Existence of a previous known SARS-CoV-2 positivity less than 1 month old (whatever the method used: RT-PCR or antigenic test)
- •SARS-CoV-2 infected participant group criteria:
- •For symptomatic subjects: onset of symptoms more than 4 days ago
- •SARS-CoV-2 uninfected participant group criteria:
- •Known history of infection and/or COVID-19 vaccination, within the previous 3 months
结局指标
主要结局
To characterize SARS-CoV-2 infection and host response, particularly mucosal immunity.
时间窗: 12 months
Measurement of mucosal anti-SARS-CoV-2 antibody levels and cytokines, composition of the local microbiota of the upper respiratory tract.
次要结局
- To characterize biomarkers of infection (stage, severity, prognosis) with new innovative direct or indirect diagnostic methods for SARS-CoV-2 and identify potential theranostic biomarkers.(12 months)
- To characterization of mucosal and humoral immunity.(12 months)
- To develop tools to assess the contamination capacity of subjects.(12 months)
- To characterize the dynamics of infection and immunological response from the date of suspected infection and up to 3 months post-infection, or later, during the first year(12 months)
- To optimize sampling techniques and calibrate the detection of viral components by artificial contamination on samples taken during the 1st visit of uninfected subjects.(12 months)