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MINI Bypass Versus Roux-en-Y Bypass: Differences in HOrmonal Secretions at 2 Years of Surgery

Completed
Conditions
Bariatric Surgery
Interventions
Biological: Blood sampling
Biological: Feces sampling
Registration Number
NCT03482895
Lead Sponsor
Assistance Publique - Hôpitaux de Paris
Brief Summary

Gastric bypass with omega loop technic (OLGB) seems to be as effective as gastric bypass roux-en-Y (RYGB the reference) for the management of obesity and type 2 diabetes, but with less early surgical complications and more undernutrition in long terms.

This study aims to explore the profile of secretion of entero-insular hormone after a meal test in OLGB patient vs RYGB to understand the mechanisms of the improvement of type 2 diabetes after OLGB.

Secondary objectives are to better understand the absorptive function of the gut after a gastric bypass, to understand why is there more undernutrition in long term after OLGB than after RYGB.

Detailed Description

In the treatment of obesity associated with type 2 diabetes, there is still few informations comparing the gastric bypass roux-en-Y (RYGB), the reference method, and the bypass in Omega (OLGB). The effectiveness of both interventions is comparable regarding weight loss and the management of type 2 diabetes but the level of proof remains low. Nutritional deficiencies and chronic diarrhea are reported in both interventions. It seems that the OLGB would cause more malabsorption and undernutrition but the OLGB would lead to less early operative complications because it has only one gastro-jejunal anastomosis compared to RYGB which has two.

The success of the RYGB for the remission of diabetes, is partly associated with a change in secretion's profile of intestinal hormones participating in the glucose homeostasis, especially the GLP-1. There is no studies published reporting the level of secretion (fasting or postprandial) of gastrointestinal hormones after OLGB. It is important to understand how this surgery produces its effects, especially on diabetes.

MINIBHO main hypothesis is that the secretion's profile of entero-insular hormones, like GLP-1, are exacerbated after OLGB just like in RYGB, that would explain the same level of improvement of T2 diabetes after both surgery.

Kinetic study of entero-insular secretion will be assessed by some blood dosages of entero-insular hormones (GLP-1, GLP-2, Gastric inhibitory polypeptide (GIP), glicentine, Insulin, peptide C, Glucagon and glucose) in 30 patients (15 OLGB and 15 RYGB). These dosages will be made at different times after a meal test (during fasting, 15 minutes, 30 minutes, 60 minutes, 90 minutes and 120 minutes after the meal) to search a significant difference in the profile of secretion between the OLGB and RYGB patients.

Investigators also hypothesize that the editing method of the OLGB leads to a decrease in the food absorption profile compared to RYGB. To explore this hypothesis, citrulline and apolipoprotein B48 (ApoB48) levels will be measured, which reflect enterocyte function of the patients, and will be compared between patients operated with RYGB vs OLGB. Functional enterocyte mass will be correlated to the absorptive function of the gut which will be evaluated by the examination of the 24h feces.

The main objective is to determine whether the secretion profiles of entero-insular hormone during a meal are different or not in patients operated with OLGB compared to RYGB. The secondary objectives are to evaluate in these patients the absorptive function and the functional enterocyte mass.

Recruitment & Eligibility

Status
COMPLETED
Sex
All
Target Recruitment
30
Inclusion Criteria
  • Roux en Y gastric bypass or omega loop gastric bypass at two years of the surgery (6 months more or less)
  • 18 years minimum
  • weight > 40kg
Exclusion Criteria
  • Type 1 diabetes diagnosed with the positivity of anti Glutamate Acid Decarboxylase (GAD) or anti Ia2
  • Treatment with Insulin, Glucagon Like Peptide 1 (GLP1) or anti-DiPeptidyl Peptidase-4 (DPP4) during the study
  • Unbalanced type 2 diabetes : HbA1c > 8%
  • Anemia with hemoglobin < 7g/dl
  • Gut inflammatory disease ; infectious disease, cancer, iatrogenic disease, auto-immune disease
  • Pregnant or breastfeeding women
  • allergy to Carmin (E120)
  • participation to another clinical trial
  • patient under legal protection

Study & Design

Study Type
OBSERVATIONAL
Study Design
Not specified
Arm && Interventions
GroupInterventionDescription
Patient: Blood sampling & Feces samplingBlood samplingBlood samplings at different times after a meal test: 0, 15, 30, 60, 90 and 120 minutes. Feces sampling: collection during 24 hours
Patient: Blood sampling & Feces samplingFeces samplingBlood samplings at different times after a meal test: 0, 15, 30, 60, 90 and 120 minutes. Feces sampling: collection during 24 hours
Primary Outcome Measures
NameTimeMethod
Kinetic of GLP-1 secretion0, 15, 30, 60, 90 and 120 minutes after a meal test

Area under the plasma concentration versus time curve of GLP-1

Secondary Outcome Measures
NameTimeMethod
Plasmatic dosage of citrullineBaseline

Plasmatic concentration of Citrulline versus enterocyte mass function

Plasmatic dosage of Apolipoprotein B48Baseline

Plasmatic concentration of Apolipoprotein B48 versus enterocyte mass function

Protein Absorptive Coefficient1 day

Percentage between protein food intakes and protein excretion in feces

kinetic of GLP-2 secretion0, 15, 30, 60, 90 and 120 minutes after a meal test

Area under the plasma concentration versus time curve of GLP-2

Lipidic Absorptive Coefficient1 day

Percentage between lipidic food intakes and lipidic excretion in feces

Glucidic Absorptive Coefficient1 day

Percentage between carbohydrate food intakes and carbohydrate excretion in feces

Kinetic of GIP secretion0, 15, 30, 60, 90 and 120 minutes after a meal test

Area under the plasma concentration versus time curve of GIP

Kinetic of Glicentin secretion0, 15, 30, 60, 90 and 120 minutes after a meal test

Area under the plasma concentration versus time curve of Glicentin

Kinetic of Peptide C secretion0, 15, 30, 60, 90 and 120 minutes after a meal test

Area under the plasma concentration versus time curve of Peptide C

Kinetic of Insulin secretion0, 15, 30, 60, 90 and 120 minutes after a meal test

Area under the plasma concentration versus time curve of Insulin

Kinetic of Glucagon secretion0, 15, 30, 60, 90 and 120 minutes after a meal test

Area under the plasma concentration versus time curve of Glucagon

Kinetic of Glycemia0, 15, 30, 60, 90 and 120 minutes after a meal test

Area under the plasma concentration versus time curve of glucose

Trial Locations

Locations (1)

AP-HP - Hôpital Européen Georges-Pompidou

🇫🇷

Paris, Île-de-France, France

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