Impact of Aging on Gastrointestinal Barrier Function During Hyperthermia
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 22
- 试验地点
- 1
- 主要终点
- Log Lactulose/Rhamnose Following Control Trial
研究概览
简要总结
The purpose of this study is to assess the gastrointestinal responses of the elderly and younger populations during hyperthermia.
详细描述
Heat waves are lethal and cause a disproportionate number of deaths in the elderly relative to any other age group. Although gastrointestinal barrier dysfunction is a primary cause of heat related illness, little is known about the effects of aging on gastrointestinal barrier function during hyperthermia. The central hypothesis of this work is that the elderly exhibit greater gastrointestinal barrier dysfunction during hyperthermia. Participants will complete a control trial where gastrointestinal permeability (without heating) will be assessed in young and older adults. In the experimental trial, controlled hyperthermia will be achieved using a water perfused, tube lined suit. Core body temperature will be raised to a maximum of 2 degrees Celsius above the baseline value, or an absolute temperature of 39.5 degrees Celsius. Core temperature will not be collected during the control trial. Comprehensive assessments of gastrointestinal barrier function and systemic inflammation will be assessed in young and older adults. The expected outcome of this work will re-shape our understanding of the consequences of aging on gastrointestinal barrier function during heat waves.
研究设计
- 研究类型
- Interventional
- 分配方式
- Non Randomized
- 干预模型
- Parallel
- 主要目的
- Basic Science
- 盲法
- None
入排标准
- 年龄范围
- 18 Years 至 —（Adult, Older Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •Healthy male and female individuals
- •18-35 years or 65+ years of age
- •Free of any underlying moderate to serious medical conditions
排除标准
- •Known heart disease
- •Any chronic medical conditions requiring regular medical therapy including cancer, diabetes, uncontrolled hypertension, inflammatory bowel disease, and uncontrolled hypercholestrolmia etc;
- •Abnormality detected on routine screening suggestive of provocable ischemia or previously undetected cardiac disease or resting left bundle branch block on screening electrocardiogram.
- •Current smokers, as well as individuals who regularly smoked within the past 3 years
- •Subject with a body mass index ≥35 kg/m2
- •Use of immunosuppressant drugs within last 4 weeks prior to screening
- •Use of antibiotics or antimicrobial medication in last month
- •Any previous abdominal surgery
- •Use of steroids in last 6 weeks
- •Regular use of probiotics in last month
- •Use of laxatives or anti-diarrhetic in last month
研究组 & 干预措施
Older participants
Individuals aged over 65 years.
干预措施: Hyperthermia Trial (Other)
Older participants
Individuals aged over 65 years.
干预措施: Control Trial (Other)
Younger participants
Individuals aged 18-39 years.
干预措施: Hyperthermia Trial (Other)
Younger participants
Individuals aged 18-39 years.
干预措施: Control Trial (Other)
结局指标
主要结局
Log Lactulose/Rhamnose Following Control Trial
时间窗: "Visit 1": 3-5 hours after ingestion of multiple sugar drink.
In vivo gastrointestinal permeability measured by quantities of orally ingested multi-sugar drink excreted in urine. The urinary recovery of each ingested sugar (lactulose, sucrose and rhamnose) was determined by multiplying the measured concentration of each sugar by the total volume of urine collected and dividing by the dose administered. Since lactulose is degraded in the colon, we used the ratio urine lactulose to rhamnose (L/R) to determine small intestinal barrier permeability.
Log Lactulose/Rhamnose Following Hyperthermia Trial
时间窗: "Visit 2": 3-5 hours after ingestion of multiple sugar drink.
In vivo gastrointestinal permeability measured by quantities of orally ingested multi-sugar drink excreted in urine. The urinary recovery of each ingested sugar (lactulose, sucrose and rhamnose) was determined by multiplying the measured concentration of each sugar by the total volume of urine collected and dividing by the dose administered. Since lactulose is degraded in the colon, we used the ratio urine lactulose to rhamnose (L/R) to determine small intestinal barrier permeability.
Log Sucrose Following Control Trial
时间窗: "Visit 1": 3-5 hours after ingestion of multiple sugar drink.
The urinary recovery of sucrose was determined by multiplying the measured concentration of sucrose by the total volume of urine collected and dividing by the dose administered. Sucrose is broken down rapidly in the duodenum, thus we used the urinary excretion of sucrose to assess and gastroduodenal permeability.
Log Sucrose Following Hyperthermia Trial
时间窗: "Visit 2": 3-5 hours after ingestion of multiple sugar drink.
The urinary recovery of sucrose was determined by multiplying the measured concentration of sucrose by the total volume of urine collected and dividing by the dose administered. Sucrose is broken down rapidly in the duodenum, thus we used the urinary excretion of sucrose to assess and gastroduodenal permeability.
次要结局
- Change in Core Temperature During Hyperthermia Trial("Visit 2": At 0 minutes and 50-90minutes into the hyperthermia trial.)
研究者
Craig Crandall
Professor
University of Texas Southwestern Medical Center