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HE4 in the Diagnostic Approach of Endometrial Cancer in Patients With Postmenopausal Bleeding

Conditions
Postmenopause
Uterine Hemorrhage
CA 125 Antigen
Endometrial Neoplasms
Cancer Biomarker
Registration Number
NCT04867109
Lead Sponsor
Nantes University Hospital
Brief Summary

This study aims to explore the sensitivity of an innovative marker, HE4, in the diagnosis approach of endometrial cancer in case of postmenopausal bleedings.

Detailed Description

Endometrial cancer is the most common pelvic gynecological cancer in France, ranking 5th among cancers in women in terms of incidence.

The most frequent symptom is post-menopausal bleeding and is one of the primary reasons for consultation in gynecological emergencies.

The diagnosis of endometrial cancer is made by histological analysis of endometrial tissue taken during a surgical intervention. The latter is mostly reassuring.

These interventions are often performed in mild situations and there is currently no element to be sufficiently reassuring to avoid surgery.

At present, there is no biological marker used in current practice. In the population of patients with post-menopausal bleeding requiring surgical exploration for diagnosis, the pathology results are often reassuring. These procedures could have been avoided, especially as these patients often have numerous co-morbidities and these surgeries are therefore more risky. The appearance of a new tumour marker could be useful in the management of these patients and avoid many unnecessary and risky surgeries

This study aims to explore the sensitivity of an innovative marker, HE4, in the diagnosis approach of endometrial cancer in case of postmenopausal bleedings.

Recruitment & Eligibility

Status
UNKNOWN
Sex
Female
Target Recruitment
100
Inclusion Criteria
  • Patient with postmenopausal bleeding
  • Patient requiring hysteroscopy or hysterectomy
  • No objection from the patient to participate in the study
Exclusion Criteria
  • Non-menopausal patient
  • Patient under guardianship, curatorship or deprived of her freedom
  • Patient with proven metastases on imaging
  • Patient with a macroscopically suspicious cervix
  • Patient presenting an ovarian cyst or an associated adnexal pathology
  • Patient contraindicated for surgical treatment (therefore not eligible for pathological analysis)
  • Patient who has already been treated with hormone therapy for breast cancer
  • Patient who has already had surgery for this pathology, with a contributing anatomopathological result (we will therefore include patients who would not benefit from an operative hysteroscopy after performing a cornier® pipelle that does not allow a positive result)

Study & Design

Study Type
OBSERVATIONAL
Study Design
Not specified
Primary Outcome Measures
NameTimeMethod
To evaluate the sensitivity of the HE4 marker in patients with postmenopausal bleeding in the diagnosis of endometrial cancerUntil the pathological results (About 10-15 days)

The HE4 assays will be performed using the electrochemiluminescence (ECL) technique on Cobas Pro module E801, Roche Diagnostics. The HE4 assay is based on a sandwich-type electrochemiluminescence method. The HE4 assay with the Roche Diagnostics Elecsys reagent has been standardized against the Fujirebo Diagnostics HE4 EIA method.

Positive/negative HE4 result for the estimation of the sensitivity of HE4 in the diagnosis of endometrial cancer.

The commonly accepted threshold for ovarian cancer management of 140pmol/l in postmenopausal patients should be used.

The gold standard for endometrial cancer is the pathological result (absence/presence of cancer) of the sample taken.

Secondary Outcome Measures
NameTimeMethod
Assess other diagnostic parameters (specificity, PPV, NPV) of HE4Until the pathological results (About 10-15 days)

ROC curve for the HE4 marker in the diagnosis of endometrial cancer.

Establish the optimal threshold of HE4 for the diagnosis of endometrial cancer (use of an ROC curve)Until the pathological results (About 10-15 days)

Search for the threshold with the best specificity of HE4 in the diagnosis of endometrial cancer

Evaluate the diagnostic capabilities of CA125 alone and in combination with HE4, as well as the REM and REM-B algorithms for the diagnosis of endometrial cancerUntil the pathological results (About 10-15 days)

Estimation of sensitivity, specificity, PPV and NPV in the diagnosis of endometrial cancer for the CA125 biomarker and the REM and REM B algorithms

Establish the existence of thresholds for HE4 and/or CA125 markers predictive of disease severity (FIGO stage)Until the results of the extension assessment in the event of proven endometrial cancer (1 month)

Search for HE4 and CA125 marker thresholds to assess disease severity by FIGO stage

Reassess the pathological threshold value of endometrial thickness on ultrasoundUntil the pathological results (About 10-15 days)

Search for the pathological threshold of endometrial thickness in the diagnosis of endometrial cancer

Assess the relationship between endometrial thickness on ultrasound and HE4 and CA125 marker valuesUntil the pathological results (About 10-15 days)

Estimation of the relationship between CA125 and HE4 markers and endometrial thickness on ultrasound

To evaluate the diagnostic capabilities of HE4 and CA125 in subgroups of smoking patients and patients with renal failureUntil the pathological results (About 10-15 days)

Estimation of sensitivity, specificity, PPV and NPV in the diagnosis of endometrial cancer for HE4 and CA125 in patients with active smoking and/or renal failure

Identify potential confounding factors associated with the value of the HE4 marker like treatments, comorbidities (renal insufficiency, high BMI) and others criteria that are collected in the medical filesUntil the pathological results (About 10-15 days)

Analyses of variables measured at inclusion that may influence the value of the HE4 marker.

Trial Locations

Locations (2)

Saint-Nazaire Hospital

🇫🇷

Saint-Nazaire, Loire-Atlantique, France

Vendee Hospital Center

🇫🇷

La Roche-sur-Yon, Vendee, France

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