ChiCTR2000038407进行中（未招募）不适用
Function and dysfunction of granulosa cells in women under ART treatment
Reproductive Medicine Team1 个研究点 分布在 1 个国家目标入组 120 人开始时间: 2020年8月30日最近更新:
试验速览
- 阶段
- 不适用
- 状态
- 进行中（未招募）
- 发起方
- 入组人数
- 120
- 试验地点
- 1
- 主要终点
- Calcium imaging in granulosa cell primary cultures
研究概览
简要总结
- Conduct human granulosa cells primary cultures, and study granulosa cells functions in vitro including examining intracellular Ca2+ homeostasis, production of hormones (e.g. estrogen), responsiveness to gonadotrophins (e.g. FSH and LH) as well as genes and signaling pathways in human granulosa cells;
- Compare the granulosa cells functions and genes expression between women with PCOS and non-PCOS.
研究设计
- 研究类型
- 观察性研究
- 主要目的
- 析因分组（即根据危险因素或暴露因素分组）
- 盲法
- N/a
入排标准
- 年龄范围
- 18 至 60（—）
- 性别
- Female
入选标准
- •Women undergoing assisted reproductive technology (ART) treatment diagnosed of PCOS or determined as non-PCOS as below,
- •The PCOS diagnosis should be based on the Rotterdam criteria (2003), briefly: two out of the three following criteria after exclusion of other aetiologies: i)Oligo-amenorrhoea/chronic anovulation; ii) Clinical and/or biochemical hyperandrogenism; iii) Ultrasound features of polycystic ovaries;
- •Women without PCOS and with following criteria can be included for the present study as non-PCOS:
- •(1) both ovaries are present;
- •(2) menstrual cycle length is between 25 and 35 days;
- •(3) there are no current or past diseases affecting the ovaries, gonadotropin or sex steroid secretion;
- •(4) there are no clinical signs of hyperandrogenism. Causes of infertility in the non-PCOS women can include tubal blockage, pelvic adhesions, or male factor.
排除标准
- •Patient refusal;
- •Vulnerable subjects.
研究组 & 干预措施
PCOS women
collection of follicular fluid
non-PCOS women
collection of follicular fluid
结局指标
主要结局
Calcium imaging in granulosa cell primary cultures
Gene expression profile of granulosa cells
Secretome profile of follicular fluid
次要结局
- Comparison in non-PCOS cells between different in vitro treatments of the cells
- Comparison between PCOS and non-PCOS granulosa cells
研究者
研究点 (1)
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