T Lymphocyte Subsets in Ulcerative Colitis
- Conditions
- Ulcerative Colitis
- Interventions
- Diagnostic Test: Flow cytometry
- Registration Number
- NCT06352515
- Lead Sponsor
- Assiut University
- Brief Summary
1. Study the distribution of peripheral blood T lymphocyte subsets among ulcerative colitis patients.
2. Correlation of T-cell subsets to therapeutic response/ disease activity.
3. Assess the value of circulating IgG anti-Integrin αvβ6 in UC.
- Detailed Description
Ulcerative colitis (UC) is an idiopathic, chronic inflammatory disease of the large intestine, frequently involving the rectum, and characterized by chronic and recurrent mucosal inflammation and ulceration. Although its cause is not well understood, current evidence suggests innate and adaptive immunity play critical roles in its pathogenesis.
One of the main classes of immune cells that are affected by and contribute to UC is T cells. T-lymphocytes comprise a complex collection of highly differentiated T-cell subsets playing key roles in the regulation and the effector phase of the immune response. CD4+ T cells were found over-activated and proliferated in UC patients, which can induce disorders of the cytokine network and increase the occurrence of colitis.
Once intestinal pathogens or inflammatory mediators are not cleared in time, pro-inflammatory mononuclear phagocytes (MNPs) or polymorphonuclear leukocytes (PMNs) are often recruited to promote the polarization of naive CD4+ T cells into Th1, Th2, Th17, Treg and other subsets of cells.
The balances Th17/ Treg cells are important for maintaining intestinal homeostasis. Once the proportion Th17 cells increases, it often induces the production of pro-inflammatory cytokines that promote colonic inflammation, whereas Treg cells are usually secrete interleukin-10 (IL-10) and transforming growth factor-β (TGF-β) for anti-inflammatory regulations.
UC-associated inflammation is also characterized by huge number of activated B cells and plasma cells, the latter being involved in the production of cytotoxic granules, immunoglobulins, and various autoantibodies, Recent studies have highlighted a novel autoantibody against integrin αvβ6 in the serum of patients diagnosed with UC.
Recently, targeting immune cells to inhibit inflammation has become a research hotspot. Biological therapies are highly effective hallmark therapies in UC. Despite their widespread use, the impact of these agents on the composition of the adaptive immune system is largely unexplored. Knowledge on such effects in UC could clarify the mechanism of action of these therapies, provide information about the status of the adaptive immune system, and could help finding cell-based markers.
Recruitment & Eligibility
- Status
- NOT_YET_RECRUITING
- Sex
- All
- Target Recruitment
- 80
- patients with clinical diagnosis of ulcerative colitis among both sexes.
- Age >18 years Old.
- Age <18 years old.
- Patients who refuse to participate in the study.
- Patients who have other autoimmune disease.
Study & Design
- Study Type
- OBSERVATIONAL
- Study Design
- Not specified
- Arm && Interventions
Group Intervention Description Group IV Flow cytometry Age- and sex-matched healthy controls. Group II Flow cytometry Ulcerative colitis patients on non-biologic immunosuppressive drugs. Group I Flow cytometry Patients with newly diagnosed, active, untreated ulcerative colitis Group III Flow cytometry Ulcerative colitis patients on established biological treatment.
- Primary Outcome Measures
Name Time Method Correlation of T-cell subtypes to therapeutic response 3 years Determine the effect of different treatment strategies used in UC on T-lymphocyte subsets
Study the distribution of T-cell subsets among ulcerative colitis patients. 3 years Investigate and compare the distribution of different T-lymphocyte subsets among ulcerative colitis patients and healthy subjects .
- Secondary Outcome Measures
Name Time Method