Age-related Changes in Sleep-wake Regulation: Effects of Sleep Loss on Possible Molecular Markers of Sleep Need
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 29
- 主要终点
- Sleep-wake induced change in cerebral availability of mGluR5 (metabotropic glutamate receptors of subtype 5)
研究概览
简要总结
A sleep deprivation protocol combined with state-of-the-art, simultaneous positron emission tomography and magnetic resonance spectroscopy imaging will be employed to investigate the effects of sleep deprivation and aging on hypothesized molecular markers of sleep need.
研究设计
- 研究类型
- Interventional
- 分配方式
- Non Randomized
- 干预模型
- Parallel
- 主要目的
- Basic Science
- 盲法
- None
入排标准
- 年龄范围
- 20 Years 至 70 Years（Adult, Older Adult）
- 性别
- Male
- 接受健康志愿者
- 是
入选标准
- •healthy male individuals
- •age: 20-35 or 60-70 years
- •right-handed
- •non-smokers
- •moderate caffeine/alcohol consumption
- •normal BMI
排除标准
- •Sleep/circadian rhythms disturbances
- •former brain injuries with loss of consciousness and brain anomalies
- •drug intake/consumption
- •medication intake
- •cardiac pacemaker
- •neurological disorders
- •prior participation (during past 2 years) in radiological or nuclear experiment
研究组 & 干预措施
Sleep deprivation older men
Older study participants will complete four nights in the sleep laboratory, whereas they will stay awake during one night. PET brain imaging will be conducted at the same circadian time on three consecutive afternoons (prior, during and after prolonged wakefulness). Additionally, validated tests of vigilance and cognitive performance will be administered and the brain waves will be recorded in wakefulness and sleep.
干预措施: Prolonged wakefulness (Behavioral)
Sleep deprivation young men
Young study participants will complete four nights in the sleep laboratory, whereas they will stay awake during one night. PET brain imaging will be conducted at the same circadian time on three consecutive afternoons (prior, during and after prolonged wakefulness). Additionally, validated tests of vigilance and cognitive performance will be administered and the brain waves will be recorded in wakefulness and sleep.
干预措施: Prolonged wakefulness (Behavioral)
结局指标
主要结局
Sleep-wake induced change in cerebral availability of mGluR5 (metabotropic glutamate receptors of subtype 5)
时间窗: Change from baseline metabotropic glutamate receptors of subtype 5 availability after 33 hours of prolonged wakefulness and 8 hours of recovery sleep
Positron emission tomography
次要结局
- Sleep-wake induced change in waking electroencephalogram (EEG)(Change from baseline waking EEG during 33 hours of prolonged wakefulness and after 8 hours of recovery sleep)
- Sleep-wake induced change in subjective state(Change from baseline sleepiness during 33 hours of prolonged wakefulness and after 8 hours of recovery sleep)
- Sleep-wake induced change in cognitive performance(Change from baseline cognitive performance during 33 hours of prolonged wakefulness and after 8 hours of recovery sleep)
- Sleep-wake induced change in potential molecular markers of sleep need(Change from baseline microRNA, fragile X mental retardation protein and brain-derived neurotrophic factor concentrations after 33 hours of prolonged wakefulness and 8 hours of recovery sleep)
- Sleep-wake induced change in glutamate concentration in prefrontal cortex and basal ganglia(Change from baseline glutamate concentration after 33 hours of prolonged wakefulness and 8 hours of recovery sleep)
- Sleep-wake induced change in sleep electroencephalogram (EEG)(Change from all-night baseline sleep EEG in 8 hours of recovery sleep after 33 hours of prolonged wakefulness)