Bioavailability of Bioactive Compounds in Broccoli
试验速览
- 阶段
- 早期 1 期
- 状态
- 已完成
- 发起方
- 入组人数
- 7
- 试验地点
- 2
- 主要终点
- Metabolic fate of ITC of the kailan-hybrid broccoli after human comsuption
研究概览
简要总结
The aim of this study was to determine the absorption of isothiocyanates (ITC) after ingestion of kailan-hybrid broccoli through the analysis of the correspondent urinary biomarkers. Furthermore, the effect of cooking (microwave) on the mentioned metabolic fate of these ITC was studied comparing to the uncooked vegetable.
详细描述
Background Glucosinolates (sulphur-containing compounds) are hydrolyzed by the plant enzyme myrosinase to ITC, which have chemopreventive properties. No information has been reported about the metabolic fate of ITC in the kailan-hybrid broccoli, which physicochemical characteristics could greatly influence that metabolic fate. Previous studies have shown that cooking process of vegetables may vary the human absorption of the correspondent nutritional and bioactive compounds.
Research Methods & Procedures A cross-over-design study of 7 subjects after ingestion of 200-g raw or microwaved kailan-hybrid broccoli was conducted. Total ITC equivalents in the vegetable, urine and blood samples were determined by HPLC as the cyclocondensation product of 1,2-benzenedithiol.
研究设计
- 研究类型
- Interventional
- 分配方式
- Randomized
- 干预模型
- Crossover
- 主要目的
- Screening
- 盲法
- None
入排标准
- 年龄范围
- 22 Years 至 50 Years（Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •Subjects with a body mass index between 19 and
- •Subjects age: 22-50 years-old.
- •Good health of subjects: confirmed by medical history, physical examination and clinical laboratory analysis of urine and blood (complete blood count and liver function markers including serum glutamic-pyruvic transaminase bilirubin test and glucose-6-phosphate dehydrogenase).
排除标准
- •Tobacco smoking.
- •Use of regular medications.
- •Had taken antibiotics for 4 weeks immediately preceding the study.
- •History of kidney stones, diabetes mellitus, bleeding disorders or family history of iron overload/hemochromatosis.
研究组 & 干预措施
Raw
Broccoli,200 grams
干预措施: raw broccoli (Other)
cooked
Microwaved, 200 grams
干预措施: raw broccoli (Other)
结局指标
主要结局
Metabolic fate of ITC of the kailan-hybrid broccoli after human comsuption
时间窗: Up to 4 weeks
Glucosinolates (sulphur-containing compounds) are hydrolyzed by the plant enzyme myrosinase to isothiocyanates (ITC), which have chemopreventive properties. Previous studies have shown that cooking process of vegetables may vary the human absorption of the correspondent nutritional and bioactive compounds.
次要结局
未报告次要终点
研究者
Ginés Benito Martínez Hernández
Doctor
Universidad Politécnica de Cartagena