A RANDOMIZED CONTROL TRIAL COMPARING THE EFFICACY OF MINOCYCLINE AS LOCAL DRUG DELIVERY AND DIODE LASER DEBRIDEMENT ON THE LEVELS OF PORPHYROMONAS GINGIVALIS IN CHRONIC PERIODONTITIS:A CLINICO MICROBIOLOGICAL STUDY

Chronic periodontitis is aninfectious disease resulting in inflammation within the supporting tissues ofthe teeth, progressive attachment loss, and bone loss.The role of Porphyromonasgingivalis is well established in the development and pathogenesis ofperiodontitis.Minocycline acts by interfering withprotein synthesis in the bacterial cell wall along with its anti-collagenaseeffect.Diode laser accelerates woundhealing, promotes angiogenesis and also augments growth factor release.

ThisRandomized Clinical Trial protocol is designed as a split mouth single maskedclinical trial of 12 weeks duration simulating the treatment flow ofconventional periodontal therapy. After qualifying for the study, 15 patientsage ranging from 30-65 years will be enrolled and 4 clinical variables alongwith the microbiological samples will be evaluated at the baseline by oneexaminer who will be recording all measurements in a single blind design. This will include Sulcus Bleeding Index (SBI)PlaqueIndex (PI), Probing Pocket depth (PPD), Clinical Attachment Level (CAL)along with subgingival plaque samples for Porphyromonas Gingivalis. Full mouth scaling and root planing will beperformed at baseline appointment following initial examination.Onequadrant will receive the diode laser therapy on the 1st, 3rd andthe 7th day after scaling and root planing. The other will receiveminocycline gel application after scaling and root planing and the gelapplication will be repeated at two and four weeks in the test sites, accordingto the manufacturer’s instructions.Subgingival plaque samples will be collected atbaseline, and at three month intervals. Sterile periodontal curette will beused for collection of subgingival plaque samples.Forsample collection the teeth surfaces will be dried with sterile gauze to avoidcontamination by saliva. Sub gingival plaque samples will be collected from deepestperiodontal pockets using sterile periodontal curette and assessed for themicrobial load using Polymerase chain reaction

This study is done to compare theefficacy of minocycline as local drug delivery and diode laser debridement andalso to evaluate the levels of Porphromonasgingivalisin chronic periodontitispatients.