Impact of Periodontal Treatment on Local miRNA Expression

Completed

• Conditions: Cardiovascular Diseases; Periodontal Diseases
• Interventions: Other: changes of gingival crevicular fluid miRNA

• Registration Number: NCT05275127
• Lead Sponsor: University of Catania

Brief Summary

To assess the impact of Fill Mouth Scaling and Root planing or Quadrant Scaling and Root planing gingival crevicular fluid (GCF) levels of miRNA 7a-5p, miRNA 21-3p, miRNA 21-5p, miRNA 100-5p, miRNA 125-5p, miRNA 200b-3p and miRNA 200b-5p and their correlation with periodontitis extent.

Detailed Description

The patients selected for the study were divided into 2 groups: group 1 (FM-SRP), group 2 (Q-SRP). Any selected subject underwent anamnestic and periodontal examination, blood and GCF sampling. MiRNA in the GCF was extracted through a commercially available kit and then measured carrying out real-time quantitative polymerase chain reaction (RT-PCR).

Study Timeline

• Study First Submitted: 2022-02-08
• Study First Submitted QC: 2022-03-01
• Study First Posted: 2022-03-11
• Study Start: 2022-03-14
• Primary Completion: 2022-11-15
• Study Completion: 2022-11-20
• Status Verified: 2023-04
• Last Update Submitted: 2023-04-06
• Last Update Posted: 2023-04-07

Recruitment & Eligibility

• Status: COMPLETED
• Sex: All
• Target Recruitment: 89

Inclusion Criteria

• Presence of at least 15 teeth

• CP with a minimum of 40% of sites with a clinical attachment level (CAL)

  ≥2mm and probing depth (PD) ≥4mm;

• Presence of at least ≥2 mm of crestal alveolar bone loss verified on digital periapical radiographs

• Presence of ≥40% sites with bleeding on probing (BOP)

Exclusion Criteria

• Intake of contraceptives
• Intake of immunosuppressive or anti-inflammatory drugs throughout the last three months prior to the study
• Status of pregnancy or lactation
• Previous history of excessive drinking
• Allergy to local anaesthetic
• Intake of drugs that may potentially determine gingival hyperplasia such as Hydantoin, Nifedipine, Cyclosporin A or similar drugs.

Study & Design

• Study Type: OBSERVATIONAL
• Study Design: Not specified

Arm && Interventions

Group	Intervention	Description
Q-SRP	changes of gingival crevicular fluid miRNA	Evaluation of changes of gingival crevicular fluid miRNA
FM-SRP	changes of gingival crevicular fluid miRNA	Evaluation of changes of gingival crevicular fluid miRNA

Primary Outcome Measures

Name	Time	Method
Clinical attachment level reduction	1-year	evaluation of millimeters reduction of clinical attachment level (CAL) after periodontal therapy

Trial Locations

Locations (1)

University of Catania; 🇮🇹 Catania, CT, Italy