The Relative Contribution of Dietary Lipids vs. of Fatty Acids to Non-adipose Tissues and the Effect of a Carbohydrate-rich Diet in Healthy Subjects
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 14
- 试验地点
- 2
- 主要终点
- whole-body organ-specific Dietary Fatty Acid (DFA) partitioning
研究概览
简要总结
The present research protocol will analyze whether a short-term modification (one week) of dietary habits would have an impact on the postprandial metabolism of dietary fatty acids and on their uptake by non-adipose tissues, in healthy subjects.
Each subject will participate in two protocols randomly determined and separated by a period of one month: a 7-day isocaloric diet (Protocol A) and a 7-day carbohydrate-rich diet containing +50% of the subject's energy needs. (Protocol B).
At the end of each diet, the subject will go through a postprandial metabolic study of 8 hours where different parameters will be measured thanks to PET imaging and perfusions of stables isotopes.
研究设计
- 研究类型
- Interventional
- 分配方式
- Randomized
- 干预模型
- Crossover
- 主要目的
- Basic Science
入排标准
- 年龄范围
- 18 Years 至 65 Years（Adult, Older Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •Healthy subjects: subjects with normal glucose tolerance determined according to an oral glucose tolerance test and with a BMI above 25 kg/m2 without first degree of familial history of type 2 diabetes (parents, siblings).
排除标准
- •overt cardiovascular disease as assessed by medical history, physical exam, and abnormal ECG
- •treatment with a fibrate, thiazolidinedione, beta-blocker or other drug known to affect lipid or carbohydrate metabolism (except statins, metformin, and other antihypertensive agents that can be safely interrupted)
- •presence of liver or renal disease, uncontrolled thyroid disorder, previous pancreatitis, bleeding disorder, or other major illness
- •smoking (>1 cigarette/day) and/or consumption of >2 alcoholic beverages per day
- •prior history or current fasting plasma cholesterol level > 7 mmol/l or fasting TG > 5 mmol/l
- •any other contraindication to temporarily interrupt current meds for lipids or hypertension
- •being pregnant
- •not be barren
研究组 & 干预措施
Isocaloric diet (7 days)
Protocole A
干预措施: PET imaging (Radiation)
Isocaloric diet (7 days)
Protocole A
干预措施: perfusions of stable tracers (Other)
Isocaloric diet (7 days)
Protocole A
干预措施: isocaloric diet (Dietary Supplement)
Isocaloric diet (7 days)
Protocole A
干预措施: Liquid meal (Other)
Isocaloric diet (7 days)
Protocole A
干预措施: Indirect calorimetry (Device)
Hypercaloric diet enriched with carbohydrate food (7 days)
Protocole B
干预措施: Liquid meal (Other)
Hypercaloric diet enriched with carbohydrate food (7 days)
Protocole B
干预措施: PET imaging (Radiation)
Hypercaloric diet enriched with carbohydrate food (7 days)
Protocole B
干预措施: perfusions of stable tracers (Other)
Hypercaloric diet enriched with carbohydrate food (7 days)
Protocole B
干预措施: Indirect calorimetry (Device)
Hypercaloric diet enriched with carbohydrate food (7 days)
Protocole B
干预措施: Hypercaloric diet (Dietary Supplement)
结局指标
主要结局
whole-body organ-specific Dietary Fatty Acid (DFA) partitioning
时间窗: 2 months
will be determined by whole-body CT (16 mA) followed by PET acquisition of 18FTHA
Left ventricular function by Positron Emiting Positron (PET) ventriculography
时间窗: 2 months
will be determined using 11C-acetate PET/CT. 180 MBq will be administered by bolus injection at fasting. After a transmission scan and regional CT (40mA), a 30-min dynamic list-mode PET acquisition will be performed on a 18 cm-high thoraco-abdominal segment to include the left cardiac ventricle and most of the liver on a Philips Gemini TOF PET/CT
次要结局
- Cardiac and hepatic oxidative metabolism index(2 months)
- metabolites appearance rate(6 months)
- Insulin secretion rate(4 months)
- β-cell function(4 months)
- Anthropometric parameters(2 months)
- energy metabolism (whole body production)(4 months)
- Insulin sensitivity(4 months)
- Cardiac DFA uptake(2 months)
- Cardiac and hepatic blood flow(2 months)
- hormonal responses(4 months)
研究者
André Carpentier
tenured professor
Université de Sherbrooke