The Significance of T-follicular Helper and T-follicular Regulatory Cells in Autoimmune Haemolytic Anemia
试验速览
- 阶段
- 不适用
- 状态
- 尚未招募
- 入组人数
- 50
- 主要终点
- The proportion of circulating T-follicular helper and T-follicular regulatory in patients with Autoimmune Hemolytic Anemia (AIHA), at diagnosis and after treatment
研究概览
简要总结
we study the circulating T-follicular regulatory and T-follicular regulatory cells in autoimmune hemolytic anemia.
详细描述
Autoimmune hemolytic anemia (AIHA) is an acquired autoimmune disease resulting in the production of antibodies directed against the patient's red blood cells (RBCs) causing shortened erythrocyte lifespan.
The main pathogensis of the disease is autoantibodies (Ab) that directed against erythrocytes, with or without complement (C) activation. The most common form of AIHA is warm AIHA characterized by the presence of warm-type autoantibodies-immunoglobulin G (IgG) which reacts optimally at 37 °C, causing RBC extravascular destruction by tissue macrophages .
Previous studies of the etiology and pathogenesis of AIHA have focused on the autoreactive B cells that have escaped tolerance mechanisms and regulatory T cells (Treg).
The main treatment of AIHA includes RBC transfusion and immune system inhibitors such as corticosteroids. During an immune response, CD4 Th cells can differentiate into several unique effector lineages that promote different immune responses via the secretion of distinct types of cytokines.
T follicular helper (Tfh) cells are a CD4 T cell lineage whose major function is to help B cells form germinal centers (GCs) and produce high-affinity antibodies(6). Tfh cells are characterized by expression of the CXCR5, the transcriptional repressor B cell lymphoma 6 (Bcl-6), programmed death 1 (PD-1), and inducible costimulator (ICOS).
研究设计
- 研究类型
- Observational
- 观察模型
- Case Control
- 时间视角
- Retrospective
入排标准
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •Patients diagnosed as autoimmune hemolytic anemia, primary or secondary, male or female, any age but not on treatment
排除标准
- •Pregnancy or breast-feeding women
- •Patients treated with corticoids or immunosuppressant.
- •Patients with hematological malignance.
- •Patients with alloimmune hemolytic anemia as ABO incompatibility or transplant patients
结局指标
主要结局
The proportion of circulating T-follicular helper and T-follicular regulatory in patients with Autoimmune Hemolytic Anemia (AIHA), at diagnosis and after treatment
时间窗: Baseline
次要结局
- Measure the ratio between the T- follicular helper and T-follicular regulatory(Baseline)