Serum Proteomics Analysis for Dynamic Changes of Differential Proteins in Sepsis Patient With iTRAQ Labeling and LC-MS/MS
试验速览
- 阶段
- 不适用
- 发起方
- 入组人数
- 100
- 试验地点
- 1
- 主要终点
- Survival status
研究概览
简要总结
Serum proteomics is a very useful tool to identify various disease. The purpose of the present study was to find differential proteins among patient with normal, SIRS, sepsis, severe sepsis, death and to screen potential biomarkers for their dynamic changes. Serum proteins were identified by iTRAQ labeling and LC-MS/MS. The bioinformatics analysis was performed with the Mascot software and the International Protein Index (IPI) and the Gene Ontology (GO) Database and KEGG pathway Database. The differentially expressed proteins were verified by Western blot by another sample collected from clinical.
研究设计
- 研究类型
- Observational
- 时间视角
- Prospective
入排标准
- 年龄范围
- 18 Years 至 —（Adult, Older Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •Male and female aged 18 years old and over;
- •clinically confirmed infection;
- •fulfilled at least two criteria of systemic inflammatory response syndrome
- •(a) core temperature higher than 38 °C or lower than 36 °C
- •(b)respiratory rate above 20/min, or PCO2 below 32 mmHg
- •(c) pulse rate above 90/min, and
- •(d) white blood cell count greater than 12,000/μl or lower than < 4,000/μl or less than 10% of bands.
排除标准
- •younger than 18 years of age;
- •acquired immunodeficiency syndrome;
- •reduced polymorphonuclear granulocyte counts (< 500 μL-1);
- •died within 24h after admission into the ICU, or refused to participate in the study, or declined treatment during the period of observation.
结局指标
主要结局
Survival status
时间窗: 28 days after admission to ICU
The survival time of patients more than 28days is defined as survival. The survival time of patients less than 28days is defined as death.
次要结局
未报告次要终点