Resveratrol-mediated Changes in Extracellular Vesicle microRNA Cargo as a Novel Contributor to Resistance Training-induced Skeletal Muscle Adaptations in Older Adults
Trial Snapshot
- Phase
- Not Applicable
- Status
- Recruiting
- Sponsor
- Texas Tech University
- Enrollment
- 36
- Locations
- 1
- Primary Endpoint
- Circulating extracellular vesicle microRNA profile
Study Overview
Brief Summary
The main goal of this study is to find out if supplementing with resveratrol improves skeletal muscle adaptations to strengthening exercise (resistance training or strength training) in adults age 60+, and to determine whether circulating vehicles of inter-organ communication (extracellular vesicles) and their cargo might help explain those responses. The investigators will also examine whether resveratrol supplementation improves blood vessel function and cognitive function (brain health) in response to strength training.
Detailed Description
After screening and providing informed consent, participants (healthy older adults, age 60+, minimally active) will be familiarized with the procedures and undergo pre-testing. After pre-testing, participants will be assigned to supplement with resveratrol or placebo (double-blinded) daily while undergoing a strength training intervention. Strength training will be performed 3x/week for two 6-week blocks, and each visit will be supervised by a member of the study team. An additional week of testing will follow each of the two 6-week blocks.
Study Design
- Study Type
- Interventional
- Allocation
- Randomized
- Intervention Model
- Parallel
- Primary Purpose
- Prevention
- Masking
- Triple (Participant, Investigator, Outcomes Assessor)
Eligibility Criteria
- Ages
- 60 Years to — (Adult, Older Adult)
- Sex
- All
- Accepts Healthy Volunteers
- Yes
Inclusion Criteria
- •Minimally active (less than 1 hour of structured exercise/week other than walking)
- •No resistance training for the previous 6 months
- •Not currently taking resveratrol supplements
- •Stable medication, supplement, dietary, and physical activity regimens for the previous 3 months and no plans to change throughout the study period
- •Body mass at least 110 lbs
Exclusion Criteria
- •Medical complications that could increase risk during exercise (e.g., cardiovascular disease, uncontrolled hypertension, type 1 or 2 diabetes, orthopedic contraindication to exercise, kidney disease, etc.)
- •Cannabis or nicotine use
- •Consume more than 7 alcohol-containing beverages per week
Arms & Interventions
Resveratrol (RSV) group
Participants will be randomized to consume resveratrol (500 mg) daily for 14 weeks (two 6-week blocks of resistance training [supervised, full-body, 3 days/week] + one testing week [3 days] following each block) after the pre-testing period. Participants will be provided with 7 days' worth of supplements at a time in pre-filled pill boxes. Pill boxes will be refilled weekly and adherence monitored.
Intervention: Resistance training (Behavioral)
Resveratrol (RSV) group
Participants will be randomized to consume resveratrol (500 mg) daily for 14 weeks (two 6-week blocks of resistance training [supervised, full-body, 3 days/week] + one testing week [3 days] following each block) after the pre-testing period. Participants will be provided with 7 days' worth of supplements at a time in pre-filled pill boxes. Pill boxes will be refilled weekly and adherence monitored.
Intervention: Resveratrol 500 mg oral once daily. (Dietary Supplement)
Placebo
Participants will be randomized to consume placebo capsules (cellulose, visually identical to resveratrol capsules) daily for 14 weeks (two 6-week blocks of resistance training [supervised, full-body, 3 days/week] + one testing week [3 days] following each block) after the pre-testing period. Participants will be provided with 7 days' worth of supplements at a time in pre-filled pill boxes. Pill boxes will be refilled weekly and adherence monitored.
Intervention: Resistance training (Behavioral)
Placebo
Participants will be randomized to consume placebo capsules (cellulose, visually identical to resveratrol capsules) daily for 14 weeks (two 6-week blocks of resistance training [supervised, full-body, 3 days/week] + one testing week [3 days] following each block) after the pre-testing period. Participants will be provided with 7 days' worth of supplements at a time in pre-filled pill boxes. Pill boxes will be refilled weekly and adherence monitored.
Intervention: Placebo (Dietary Supplement)
Outcomes
Primary Outcomes
Circulating extracellular vesicle microRNA profile
Time Frame: During pre-, mid-, and post-testing (each separated by about 6 weeks)
Circulating extracellular vesicles will be isolated from plasma and their microRNA cargo will be sequenced.
Muscle torque
Time Frame: During pre-, mid-, and post-testing (each separated by about 6 weeks)
Participants will be instructed to try and straighten their leg, against resistance, as forcefully as possible and maximal torque of the quadriceps assessed using a dynamometer.
Muscle fatigability
Time Frame: During pre-, mid-, and post-testing (each separated by about 6 weeks)
Participants will perform 32 repetitions of knee extensions on a dynamometer and the change in torque from the beginning to the end will be used to indicate fatigability.
Muscle strength
Time Frame: During pre-, mid-, and post-testing (each separated by about 6 weeks)
Participants will perform submaximal testing to assess strength on key exercises included in the strength training protocol.
Appendicular skeletal muscle mass index
Time Frame: During pre-, mid-, and post-testing (each separated by about 6 weeks)
Assessed using dual-energy x-ray absorptiometry (DEXA)
Secondary Outcomes
- Waist-to-hip ratio(During pre-, mid-, and post-testing (each separated by about 6 weeks))
- Vascular function(During pre-, mid-, and post-testing (each separated by about 6 weeks))
- Cognitive function(During pre-, mid-, and post-testing (each separated by about 6 weeks))
- Grip strength(During pre-, mid-, and post-testing (each separated by about 6 weeks))
- Lower body strength/endurance(During pre-, mid-, and post-testing (each separated by about 6 weeks))
- Gait speed(During pre-, mid-, and post-testing (each separated by about 6 weeks))