Exercise, Gut Microbiota in Sedentary Adults With Overweight
- Conditions
- Metabolic Disturbance
- Interventions
- Behavioral: HIIT group
- Registration Number
- NCT03515642
- Lead Sponsor
- Universidad Santo Tomas
- Brief Summary
This project offers the opportunity to obtain detailed measurements on the health of body composition, metabolic health, and intestinal microbial diversity in overweight adults; moreover to provide information about the effects of physical exercise on them. The aim of the research is to advance in the understanding of the mechanisms induced by physical exercise, which includes the measurement of nutritional parameters, clinical and biochemical biomarkers of hepatic/cardiometabolic health, as well as the application of differential proteomics technologies by means of Arrays of cytokines and intestinal microbiome, making this study a novel and pioneer in sensitive areas of primary health care in Latin America.
- Detailed Description
Not available
Recruitment & Eligibility
- Status
- WITHDRAWN
- Sex
- Male
- Target Recruitment
- Not specified
- Healthy
- Sedentary males
- BMI>25 kg/m2, on a stable body weight for the last 3 months
- Acute or chronic illness
- Use of antibiotics the past 2 months
- Smoking
Study & Design
- Study Type
- INTERVENTIONAL
- Study Design
- PARALLEL
- Arm && Interventions
Group Intervention Description HIIT group HIIT group The HIIT modality consisted of 30-40 minutes (min) of steady-state, high-intensity training 3 d/wk on a stationary bicycle at the target heart rate (HR) range equivalent to 85% to 95% of the individual's maximum oxygen consumption rate (VO2max). Exercise will be performed at three sessions per week. All sessions will be supervised by an exercise physiologist during 6-weeks. SIT group HIIT group The SIT modality consisted of 6 to 10 repetitions of a 30 s segment of all-out exercise interspersed with 2 min of recovery, 3 d/wk on a stationary bicycle at the target heart rate (HR) range equivalent to 90% to 95% of the individual's maximum oxygen consumption rate (VO2max).
- Primary Outcome Measures
Name Time Method Gut microbiota Baseline, and 6 weeks immediately after the interventions ends Change from Baseline in the gut microbiota composition measured by amplicon sequencing
Plasma metabolomic profile Baseline, and 6 weeks immediately after the interventions ends Change from Baseline in the metabolomic profile will be also evaluated by a HPLC-MS approach
- Secondary Outcome Measures
Name Time Method Glucose Baseline, and 6 weeks immediately after the interventions ends Change from Baseline will be also evaluated by a spectroscopy
Insulin Baseline, and 6 weeks immediately after the interventions ends Change from Baseline will be also evaluated by a ELISA
Plasma hepatokines Baseline, and 6 weeks immediately after the interventions ends Change from Baseline in the FGF21 (fibroblast growth factor 21) Leukocyte cell-derived will be measured using ELISA kits
Body composition Baseline, and 6 weeks immediately after the interventions ends Differences in mass muscle measured by DXA
Physical fitness Baseline, and 6 weeks immediately after the interventions ends Differences in Maximum Oxygen Consumption (VO2 max K5 Gas Analyser) in ml/kg/min
Glycated hemoglobin Baseline, and 6 weeks immediately after the interventions ends Change from Baseline will be also evaluated by a ELISA
Bioinflammatory blood markers Baseline, and 6 weeks immediately after the interventions ends Change from Baseline measure of plasma CRP concentrations measured ELISA
Energy substrate oxidation Baseline, and 6 weeks immediately after the interventions ends Fat and carbohydrate oxidation in the fasted and postprandial state measured before and after the interventions using indirect calorimetry (K5 cosmed)
Resting Metabolic Rate Baseline, and 6 weeks immediately after the interventions ends Resting metabolic rate was measured before and after the interventions (K5 Cosmed)
Trial Locations
- Locations (1)
Robinson Ramírez-Vélez
🇨🇴Bogotá, Cundinamarca, Colombia