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Targeting the Gut to Improve Seizure Control in CDKL5 Deficiency Disorder (CDD)

Not Applicable
Recruiting
Conditions
CDKL5
Interventions
Dietary Supplement: alpha-lactalbumin, fructooligosaccharides, inulin
Dietary Supplement: alpha-lactalbumin, sodium butyrate, fructooligosaccharides, inulin
Registration Number
NCT06448663
Lead Sponsor
University of Milan
Brief Summary

Standard anti-seizure medications have limited efficacy in seizure control in cyclin-dependent kinase-like 5 deficiency disorder (CDD).

The study will investigate whether targeting the gut-microbiota-brain axis in CDD patients can alleviate seizures and ameliorate other comorbidities.

Detailed Description

CDD is a neurodevelopmental condition characterized by infantile-onset epilepsy, developmental delays, intellectual and motor disabilities, sleep disturbances, and cortical visual impairment. Currently, there is no treatment for CDD, and epilepsy is a prominent and severe feature of the disorder. Standard anti-seizure medications have limited efficacy in seizure control, leading to detrimental effects on cognitive and motor development in CDD.

The gut-brain axis has gained attention in epilepsy research, prompted by evidence of gastrointestinal (GI) symptoms in people with epilepsy. Studies have demonstrated significant changes in gut microbial composition in animal models and between individuals with epilepsy and healthy subjects. Notably, CDD patients experience GI problems, and we discovered that they exhibit alterations in their gut microbiota compared to healthy individuals. The study will investigate whether supplementing CDD patients with alpha-lactalbum and prebiotics alone or with post-biotics can improve neurological features and modulate microbiota composition.

Recruitment & Eligibility

Status
RECRUITING
Sex
All
Target Recruitment
20
Inclusion Criteria

clinical diagnosis of CDD and demonstrated CDKL5 pathogenic variant; drug-resistant seizures; ensured participation of a caregiver; willingness to sign the informed consent.

Exclusion Criteria

organic GI disorders (i.e., food allergies, celiac disease); special diets; percutaneous endoscopic gastrostomy tube; use of antibiotics or probiotics in the previous month.

Study & Design

Study Type
INTERVENTIONAL
Study Design
SEQUENTIAL
Arm && Interventions
GroupInterventionDescription
CDD armalpha-lactalbumin, fructooligosaccharides, inulinThis is a 32-week pilot, single site, cross-over trial of alpha-lactalbumin+ inulin + fructo-oligosaccharides vs alpha-lactalbumin + sodium butyrate + inulin + fructo-oligosaccharides. Periods I and II of the study are 12-week long together with a 4-week washout period.
CDD armalpha-lactalbumin, sodium butyrate, fructooligosaccharides, inulinThis is a 32-week pilot, single site, cross-over trial of alpha-lactalbumin+ inulin + fructo-oligosaccharides vs alpha-lactalbumin + sodium butyrate + inulin + fructo-oligosaccharides. Periods I and II of the study are 12-week long together with a 4-week washout period.
Primary Outcome Measures
NameTimeMethod
EpilepsyChange at 3 months from baseline of each round of supplementation

to evaluate the number of CDD patients considered treatment responders (seizure reduction ≥50%, ≥75% or ≥100% from baseline in monthly seizure counts) during the 12- week treatment period in 1st and 2nd round of supplementation

Secondary Outcome Measures
NameTimeMethod
Gut microbiotaChange at 3 months from baseline of each round of supplementation

to evaluate indicator species that could help as biomarkers for guiding clinicians to choose the intervention with the most likelihood of improve patient quality of life.

Sleep disturbancesChange at 3 months from baseline of each round of supplementation

Decreased Sleep SDSC scale (max score=125) by at least 5%

gastrointestinal discomfortChange at 3 months from baseline of each round of supplementation

Decrease GISI scale (max score = 17), by at least 2 points

Trial Locations

Locations (1)

University of Milan

🇮🇹

Milan, Italy

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