Impact of Chronic Obstructive Pulmonary Disease on Peripheral and Respiratory Muscle Regeneration
试验速览
- 阶段
- 不适用
- 入组人数
- 27
- 主要终点
- Muscle satellite cell proliferation index
研究概览
简要总结
Muscle weakness and atrophy are important consequences of chronic obstructive pulmonary disease (COPD) and can affect both respiratory and peripheral muscles. Impaired muscle regeneration is thought to be implicated in the process of muscle atrophy. In this study the investigators will evaluate muscle regenerative potential of the Vastus Lateralis (VL) and diaphragm of patients with COPD, compared to patients with normal lung function.
详细描述
Preliminary results suggest that the activation and differentiation processes of satellite cells isolated from the VL of patients with COPD could be altered. The investigators propose to compare the regenerative potential of the VL and the diaphragm in 15 patients with COPD and 15 individuals with normal lung function undergoing a thoracic surgery. From muscle biopsies, satellite cells will be isolated and cultured to assess their proliferation and differentiation rates, and the expression level of myogenic effectors (MyoD, Myf5, myogenin, and MRF4). Alterations in these measurements will confirm the hypotheses that the regenerative potential is lower in both muscle groups in patients with COPD compared to controls and that in COPD, the regenerative potential is lower in VL compared to the diaphragm.
研究设计
- 研究类型
- Observational
- 观察模型
- Case Crossover
- 时间视角
- Prospective
入排标准
- 年龄范围
- 40 Years 至 80 Years（Adult, Older Adult）
- 性别
- All
- 接受健康志愿者
- 否
入选标准
- •Patient with a scheduled thoracic surgery
- •≥ 40 years
- •Smoking cessation ≥ 1 month
- •Smoking history ≥ 10 pack-year
排除标准
- •Subjects who received either chemiotherapy or radiotherapy
- •Hypoxemia
- •Body weight loss ≥ 10%
- •Use of systemic corticosteroid in the last month
结局指标
主要结局
Muscle satellite cell proliferation index
时间窗: Sequential analyses performed over a period of 2 weeks after tissue collection.
Isolation from muscle biopsies of satellite cell to evaluate their regeneration capacity by evaluating the proliferation capacity
Muscle satellite cell differentiation index
时间窗: Sequential analyses performed over a period of 2 months after tissue collection.
Isolation from muscle biopsies of satellite cell to evaluate their regeneration capacity by evaluating the differentiation capacity
次要结局
未报告次要终点