Immunoblot and Chemiluminescence Assays Versus ImmunoCap: Evaluation of Allergen Specific IgE in Egyptian House Dust Mites Allergic Rhinitis Patients
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 84
- 试验地点
- 2
- 主要终点
- Reduction of serum specific IgE
研究概览
简要总结
In the present study the investigators assay house dust mites (HDMs) IgE in sera of allergic rhinitis patients (AR) using immunoblotting, chemiluminescence, and ImmunoCAP assays to compare between the accuracy of both immunoblotting and chemiluminescence assays, and ImmunoCAP as gold standard.
详细描述
All allergic rhinitits patients who were monosensitized to mixed HDMs, identified by SPT, will be subjected to complete history taking and assessment of the inclusion and exclusion criteria, performance of skin prick test and measurement of total serum IgE, measurement of specific IgE by Chemiluminescence Immunoassa, immunoblot assay and ImmunoCAP™ Phadiatop™. The patients will be followed-up for 6 months.
研究设计
- 研究类型
- Interventional
- 分配方式
- Na
- 干预模型
- Single Group
- 主要目的
- Diagnostic
- 盲法
- None
入排标准
- 年龄范围
- 20 Years 至 50 Years（Adult）
- 性别
- All
- 接受健康志愿者
- 否
入选标准
- •Patient consent.
- •Patient with (18-40) years age known to be allergic rhinitis.
- •Patient with IgE mediated allergic rhinitis (positive total IgE).
- •Patient with positive skin prick testing and monosensitized to HDM
排除标准
- •Patient refusal
- •Patients taking oral corticosteroid.
- •Patients taking anti-IgE injections.
- •Patients taking intranasal corticosteroid.
- •Patient with non- IgE mediated allergic rhinitis.
- •Patients with chronic diseases.
结局指标
主要结局
Reduction of serum specific IgE
时间窗: 6 months from diagnosis to end of Follow-up period
Improvement of symptoms and signs of allergy
Conversion of SPT to negative
时间窗: 6 months from diagnosis to end of Follow-up period
Improvement of symptoms and signs of allergy
次要结局
未报告次要终点
研究者
Noha M Hammad, MD
Associate Professor
Zagazig University