Antibacterial Effectiveness of Two Root Canal Irrigants in Root-filled Teeth With Infection: A Randomized Clinical Trial
试验速览
- 阶段
- 2 期
- 状态
- 已完成
- 入组人数
- 49
- 试验地点
- 1
- 主要终点
- Periapical radiolucency (PAI- and Friedman-scores)
研究概览
简要总结
The aim of this randomized clinical trial is to compare the antibacterial effects of 1% sodium hypochlorite (NaOCl) and 2% chlorhexidine gluconate (CHX) during retreatment of teeth with apical periodontitis (AP).
详细描述
Root canal-treated teeth with AP were randomly distributed in two groups according to the irrigant used. After removal of the previous filling material, bacteriological samples were taken from the canals before (S1) and after chemomechanical preparation using either NaOCl or CHX (S2), and after calcium hydroxide medication for 2-3 weeks (S3). 16S rRNA gene-based real-time qPCR was performed to detect and quantify total bacteria, Streptococcus species and Enterococcus faecalis.
研究设计
- 研究类型
- Interventional
- 分配方式
- Randomized
- 干预模型
- Parallel
- 主要目的
- Treatment
- 盲法
- Single (Participant)
入排标准
- 年龄范围
- 20 Years 至 95 Years（Adult, Older Adult）
- 性别
- All
- 接受健康志愿者
- 否
入选标准
- •Clinical diagnosis of apical periodontitis in a root-filled tooth assessed by radiography.
排除标准
- •Presence of gross carious lesions in the diagnosed tooth,
- •Presence of fractures involving the periodontium of the diagnosed tooth,
- •Presence of periodontal pockets more than 4 mm deep in the diagnosed tooth,
- •Diagnosis of diabetes,
- •Diagnosis of HIV infection,
- •Other immunocompromising disease,
- •Antibiotic therapy within the previous 3 months.
研究组 & 干预措施
1% sodium hypochlorite (NaOCl)
Retreatment of root-filled teeth with infection with 1% NaOCl as irrigation solution.
Total bacterial counts, Streptococcal species and Enterococcus faecalis species were quantified using qPCR against 16SrRNA before irrigation (S1), after irrigation (S2), and after intracanal medication (S3).
干预措施: Retreatment of root-filled teeth with infection (Procedure)
2% chlorhexidine gluconate (CHX)
Retreatment of root-filled teeth with infection with 2% CHX as irrigation solution.
Total bacterial counts, Streptococcal species and Enterococcus faecalis species were quantified using qPCR against 16SrRNA before irrigation (S1), after irrigation (S2), and after intracanal medication (S3).
干预措施: Retreatment of root-filled teeth with infection (Procedure)
结局指标
主要结局
Periapical radiolucency (PAI- and Friedman-scores)
时间窗: 12 months
次要结局
- Pyrosequencing of species found before and after the intervention(21 days)
- Bacterial load assessed by qPCR(21 days)