MedPath

Digital Pathology and AI for Liver Outcomes in MASLD (DPAILO-2)

Not yet recruiting
Conditions
Metabolic Dysfunction-associated Steatotic Liver Disease
Interventions
Diagnostic Test: Digital Pathology FibroNest Phenotypic Fibrosis Composite Score (Ph-FCS)
Registration Number
NCT06493253
Lead Sponsor
PharmaNest, Inc
Brief Summary

The aim of this multi-center, retrospective epidemiologic study is to confirm the prognostic performance of the Digital Pathology (DP) FibroNest Phenotypic Fibrosis Composite Score (Ph-FCS), derived from standard digital pathology liver biopsy images, in predicting clinical hepatic decompensation events in patients with metabolic dysfunction-associated steatohepatitis (MASH).

Detailed Description

MASH (Metabolic Dysfunction-Associated Steatohepatitis):

MASH presents histological liver changes similar to those caused by alcohol abuse, but occurs in the absence of alcohol intake. It is common among adults with conditions such as obesity and type-2 diabetes. Severe MASH is expected to become a leading cause of end-stage liver disease.

Current Challenges:

There are currently no fully approved treatments for MASH which places a significant burden on liver health and transplantation. Diagnosis and assessment rely on subjective histological reviews, which are prone to variability and limitations in detecting subtle changes. Therefore, there is an urgent need for accurate and continuous histological biomarkers.

FibroNest Ph-FCS Solution:

The FibroNest Ph-FCS offers a promising solution by utilizing high-resolution digital pathology and sophisticated algorithmic methods for sensitive and reproducible fibrosis severity assessment and prediction of clinical events. In a 2003 proof-of-concept retrospective study on 400 patients, the Ph-FCS demonstrated excellent prognostic performance.

Proposed Study:

This multi-center retrospective study aims to confirm the Ph-FCS's prognostic value using patient liver biopsies and clinical outcome data from the NAFLD Adult Database 2 registry (NCT01030484). The prognostic performance of the Ph-FCS will be compared to:

1. The NASH-CRN Histological Fibrosis Stages established from the same biopsies.

2. Non-invasive biomarkers like Fib-4 and elastography/Fibroscan, also collected retrospectively from the point of initial diagnosis.

Study Objectives:

(i) Confirm the Ph-FCS's prognostic utility on a large scale. (ii) Compare the Ph-FCS's prognostic performance with that of the NASH-CRN Fibrosis Stages established from the same biopsies.

(iii) Compare biopsy-based Ph-FCS with non-invasive biomarkers.

Recruitment & Eligibility

Status
NOT_YET_RECRUITING
Sex
All
Target Recruitment
1700
Inclusion Criteria

Not provided

Exclusion Criteria

From NCT01030484

  • Clinical or histological evidence of alcoholic liver disease or alcohol consumption during the two years before entry (> 20g/day for men, >10g/day women)
  • History of total parenteral nutrition
  • History of gastric or jejunoileal bypass preceding the diagnosis of NAFLD
  • Biliopancreatic diversion or bariatric surgery
  • Evidence of advanced liver disease with Child-Pugh-Turcotte score equal to or greater than 10
  • Short bowel syndrome
  • Suspected or confirmed hepatocellular carcinoma
  • Positive for HIV
  • Evidence of HBV or HCV infection
  • Low alpha-1-antitrypsin level and ZZ phenotype
  • Wilson's disease
  • Known glycogen storage disease, dysbetalipoproteinemia, phenotypic hemochromatosis
  • Vascular lesions
  • Iron overload greater than 3+
  • Zones of confluent necrosis, infarction, massive or sub-massive, pan-acinar necrosis
  • Multiple epithelioid granulomas
  • Congenital hepatic fibrosis
  • Polycystic liver disease
  • Other metabolic or congenital liver disease

Study & Design

Study Type
OBSERVATIONAL
Study Design
Not specified
Arm && Interventions
GroupInterventionDescription
Liver Related EventDigital Pathology FibroNest Phenotypic Fibrosis Composite Score (Ph-FCS)Liver-related events include liver-related death, hepatic decompensation events (variceal hemorrhage, ascites, hepatic encephalopathy), and hepatocellular carcinoma.
Non-Liver Related EventDigital Pathology FibroNest Phenotypic Fibrosis Composite Score (Ph-FCS)Absence of any of the liver events described in the second group in the patient clinical follow-up.
Primary Outcome Measures
NameTimeMethod
Performance of Hepatic Decompensation Event predictive value of the FibroNest Ph-FCSTime-to-event analysis between 2 and 10 years

Area under Receiver Operating Characteristic Curve (AUROC) of the FibroNest PT-Ph-FCS, as a prognostic/diagnostic biomarker for liver related events in patients with MASH.

Secondary Outcome Measures
NameTimeMethod
Performance of Hepatic Decompensation Event predictive value of the NASH-CRN Fibrosis StageTime-to-event analysis between 2 and 10 years

Area under Receiver Operating Characteristic Curve (AUROC) of the NASH-CRN Fibrosis Stage, as a prognostic/diagnostic biomarker for liver related events in patients with MASH.

Performance of Hepatic Decompensation Event predictive value of the elastography (Fibroscan) biomarker, a non-invasive testTime-to-event analysis between 2 and 10 years

Area under Receiver Operating Characteristic Curve (AUROC) of the elastography (Fibroscan) biomarker, as a prognostic/diagnostic biomarker for liver related events in patients with MASH.

Trial Locations

Locations (1)

Virginia Commonwealth University

🇺🇸

Richmond, Virginia, United States

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