The impact of fixed orthodontic appliances on the oral microbiome in adolescent and adult patients using next generation sequencing (NGS) : A longitudinal comparative study.

Summary

Aim: To evaluate and compare oral microbiome during fixed orthodontic therapy in young and adult patients and to correlate with the clinical parameters.

Objective: To evaluate oral microbiome during fixed orthodontic therapy in adolescent patients at three different observation times

**Methodology:**Study sample collection will be started afterethical clearance. For the biological sample collection. Supragingival plaque will be isolated using cotton and airdried. Supragingival plaque will be collected using a periodontal probe, and after collection, plaque will be dropped in an Eppendorf tube containing ionized molecular water and shaken until the plaque dislodged from the tip and into the tube. A sample will be collected at time intervals- before the start of treatment (T1), During the treatment (T2), and completion of treatment (T3)

Outcomes- A comparative analysis of oral microbiome between adolescent and adults may identify specific bacterial taxonomy responsible for periodontitis, gingivitis and white spot, thus aid in formulation of a targeted approach.