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A Study of CART-TnMUC1 in Patients With TnMUC1-Positive Advanced Cancers

Phase 1
Terminated
Conditions
Triple Negative Breast Cancer
Multiple Myeloma
Ovarian Cancer
Pancreatic Ductal Adenocarcinoma
Non-Small Cell Lung Cancer
Fallopian Tube Cancer
Interventions
Registration Number
NCT04025216
Lead Sponsor
Kite, A Gilead Company
Brief Summary

Multi-center, open-label, first in human Phase 1 study of the safety, tolerability, feasibility, and preliminary efficacy of the administration of genetically modified autologous T cells (CART-TnMUC1 cells) engineered to express a chimeric antigen receptor (CAR) capable of recognizing the tumor antigen, TnMUC1 and activating the T cell (CART- TnMUC1 cells).

Detailed Description

The Dose Escalation phase of the study is designed to identify the dose and regimen of CART-TnMUC1 cells that can be safely administered intravenously following the lymphodepletion (LD) regimen to patients with (1) advanced TnMUC1+ solid tumors (triple negative breast cancer, epithelial ovarian cancer, pancreatic cancer, and non-small cell lung cancer) and (2) advanced TnMUC1+ multiple myeloma in a parallel two-arm dose escalation study. The Dose Escalation phase is anticipated to enroll approximately 40 patients.

The Expansion phase of the study is designed to assess the preliminary efficacy of CART-TnMUC1 cells administered intravenously to patients with TnMUC1+ solid tumors. The Expansion phase is anticipated to enroll approximately 72 patients (18 patients per each tumor indication).

Recruitment & Eligibility

Status
TERMINATED
Sex
All
Target Recruitment
16
Inclusion Criteria
  • Confirmed diagnosis of metastatic treatment-resistant ovarian cancer (including cancers of the fallopian tube), pancreatic adenocarcinoma, hormone receptor (HR)-negative and HER2-negative (triple negative) breast cancer (TNBC) or non-small cell lung cancer (NSCLC), or relapsed/refractory multiple myeloma

  • Eastern Cooperative Oncology Group (ECOG) score of 0 or 1

  • Prior therapies as defined by tumor type:

    • Multiple myeloma: relapsed or refractory disease previously treated with or intolerant to at least three different lines of therapy that contained at least one of the following drug classes: proteasome inhibitor, an immune modulatory drug, alkylators, CD38 monoclonal antibody and glucocorticoids. Patients must be at least 90 days since autologous stem cell transplant
    • NSCLC: i.) Patients without driver mutations must have received standard therapy, including both checkpoint inhibition and platinum-based chemotherapy or be intolerant of these standard therapies ii.) Patients with driver mutations must have received or be intolerant to prior targeted therapy directed at the specific identified mutations in addition to the standard chemotherapy classes
    • Pancreatic adenocarcinoma: disease progression following at least one standard of care systemic chemotherapy for metastatic or unresectable disease or be intolerant of these standard therapies
    • TNBC: ER and/or PR < or =10%, HER2 negative and experienced disease progression following at least one prior systemic anti-cancer therapy regimen as part of their treatment for management of metastatic breast cancer or be intolerant to these standard therapies
    • Ovarian: platinum-resistant (progression of disease by either CA-125, clinical or radiographic assessment within 6 months of last platinum-based chemotherapy ) and must have received at least two prior lines of therapy for metastatic ovarian cancer, including at least one prior line of therapy including a platinum-containing regimen or be intolerant of these standard therapies
  • Evaluable disease as defined by tumor type

  • TnMUC1+ disease, determined by centrally tested TnMUC1 expression in a prior or archival tumor biopsy

  • Toxicities from any previous therapy must have recovered to Grade 1 or baseline

  • Estimated estimated glomerular filtration rate ≥ 60 m/min by Modification of Diet in Renal Disease criteria

  • Aspartate aminotransferase (AST) or alanine aminotransferase (ALT) ≤ 2.5 x upper institutional limit of normal with the following exception: Patients with known hepatic metastases, AST or ALT ≤ 3 x upper institutional limit of normal

  • Serum total bilirubin < 1.5 mg/dL with the following exception: patients with known Gilbert's disease, serum total bilirubin < 3 mg/dL

  • Serum albumin ≥ 3.0 g/dL (solid tumor patients in Arm 1 and Phase 1a only, not applicable to patients with multiple myeloma)

  • Left ventricular ejection fraction (LVEF) ≥ 50%. LVEF assessment must have been performed within 6 months of treatment start

  • Hemoglobin ≥ 8 g/dL

  • Absolute neutrophil count ≥ 1000/μL

  • Platelet count ≥ 75,000/μL (for Multiple Myeloma patients with bone marrow plasma cells ≥ 50% of cellularity: ≥ 30,000/μL)

  • Patients of reproductive potential agree to use approved contraceptive methods per protocol

Key

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Exclusion Criteria
  • Active invasive cancer other than the proposed cancers included in the study
  • Current treatment with systemic high-dose corticosteroids (defined as a dose greater than the equivalent of prednisone 10 mg/day)
  • Active autoimmune disease (including connective tissue disease, uveitis, sarcoidosis, inflammatory bowel disease or multiple sclerosis) or have a history of severe autoimmune disease requiring prolonged immunosuppressive therapy (any immunosuppressive therapy should have been stopped within 6 weeks prior to screening visit)
  • Current human immunodeficiency virus (HIV), hepatitis C virus (HCV), hepatitis B virus (HBV) infections
  • Other active or uncontrolled medical or psychiatric condition that would preclude participation in the opinion of the Sponsor or Principal Investigator
  • Prior allogeneic stem cell transplant
  • Active and untreated central nervous system (CNS) malignancy
  • History of severe infusion reaction to monoclonal antibodies or biological therapies, or to study product excipients that would preclude the patient safely receiving CART-TnMUC1 cells
  • Active or recent (within the past 6 months prior to apheresis) cardiovascular disease, defined as (1) New York Heart Association (NYHA) Class III or IV heart failure, (2) unstable angina or (3) a history of recent (within 6 months) myocardial infarction or sustained (> 30 second) ventricular tachyarrhythmias, or (4) cerebrovascular accident
  • Have inadequate venous access for or contraindications for the apheresis procedure
  • Pregnant or breastfeeding women
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Study & Design

Study Type
INTERVENTIONAL
Study Design
SEQUENTIAL
Arm && Interventions
GroupInterventionDescription
Dose Escalation Arm1: Solid TumorsCART-TnMUC1Intravenous CART-TnMUC1 cells for patients with TnMUC1+ treatment-resistant ovarian cancer (including cancers of the fallopian tube), pancreatic ductal adenocarcinoma, hormone receptor (HR)-negative and human epidermal growth factor receptor 2 (HER2)-negative (triple negative) breast cancer and non-small cell lung cancer
Dose Escalation Arm 2: Multiple MyelomaCART-TnMUC1Intravenous CART-TnMUC1 cells for patients with TnMUC1+ relapsed/refractory multiple myeloma
Dose Escalation Arm1: Solid TumorsFludarabineIntravenous CART-TnMUC1 cells for patients with TnMUC1+ treatment-resistant ovarian cancer (including cancers of the fallopian tube), pancreatic ductal adenocarcinoma, hormone receptor (HR)-negative and human epidermal growth factor receptor 2 (HER2)-negative (triple negative) breast cancer and non-small cell lung cancer
Dose Escalation Arm1: Solid TumorsCyclophosphamideIntravenous CART-TnMUC1 cells for patients with TnMUC1+ treatment-resistant ovarian cancer (including cancers of the fallopian tube), pancreatic ductal adenocarcinoma, hormone receptor (HR)-negative and human epidermal growth factor receptor 2 (HER2)-negative (triple negative) breast cancer and non-small cell lung cancer
Dose Escalation Arm 2: Multiple MyelomaCyclophosphamideIntravenous CART-TnMUC1 cells for patients with TnMUC1+ relapsed/refractory multiple myeloma
Dose Escalation Arm 2: Multiple MyelomaFludarabineIntravenous CART-TnMUC1 cells for patients with TnMUC1+ relapsed/refractory multiple myeloma
Primary Outcome Measures
NameTimeMethod
Cohort Expansion: Objective Response in solid tumorsUp to 2 years

Proportion of patients having a confirmed Complete Response (CR) or Partial Response (PR) per Response Evaluation Criteria in Solid Tumors (RECIST) v1.1

Dose Escalation: Dose Identification of CART-TnMUC1Up to 2 years

Incidence of Dose Limiting Toxicity (DLT) in solid tumors and multiple myeloma

Secondary Outcome Measures
NameTimeMethod
Preliminary anti-tumor efficacy of CART as assessed by Time to Progression (TTP) in multiple myelomaUp to 2 years

Time to Progression as defined by the interval between CART-TnMUC1 cell infusion and first documented progression or death due to disease per RECIST v1.1 in solid tumors and IMWG criteria in multiple myeloma

Preliminary anti-tumor efficacy of CART as assessed by Minimal Residual Disease (MRD) in multiple myelomaUp to 2 years

Defined by MRD-negative rate per IMWG criteria

Preliminary anti-tumor efficacy of CART as assessed by Overall Survival (OS) in solid tumors and multiple myelomaUp to 2 years

OS as defined by the interval between CART-TnMUC1 cell infusion and date of death by any cause

Preliminary anti-tumor efficacy of CART as assessed by Duration of Response (DOR) in solid tumors and multiple myelomaUp to 2 years

DOR as defined by the interval of first documented response until first documented disease progression or death in solid tumors and multiple myeloma

Preliminary anti-tumor efficacy of CART as assessed by Progression Free Survival (PFS) in solid tumorsUp to 2 years

PFS as defined by the interval between CART-TnMUC1 cell infusion and date of disease progression by RECIST v1.1 or death in solid tumors and by International Myeloma Working Group (IMWG) criteria in multiple myeloma

Preliminary anti-tumor efficacy of CART as assessed by Objective Response Rate (ORR) in solid tumors and multiple myelomaUp to 2 years

Proportion of patients having a confirmed CR or PR per RECIST v1.1 in solid tumors and IMWG criteria (PR, Very Good PR, CR, Stringent CR) in multiple myeloma

Preliminary anti-tumor efficacy of CART as assessed by Clinical Benefit Rate in solid tumorsUp to 2 years

Proportion of patients having a confirmed CR, PR, or Stable Disease (SD) per RECIST v1.1

Preliminary anti-tumor efficacy of CART as assessed by Time to Response (TTR) in solid tumors and multiple myelomaUp to 2 years

Time to Response as defined by the interval between CART-TnMUC1 cell infusion and first documented CR or PR per RECIST v1.1 in solid tumors and IMWG criteria in multiple myeloma

Peripheral expansion and persistence of CART-TnMUC1 cells in solid tumors and multiple myelomaUp to 15 years

Defined as quantitative polymerase chain reaction (qPCR) documenting loss of CART cells

Safety of CART-TnMUC1 in solid tumors and multiple myelomaUp to 2 years

Percentage of patients experiencing adverse events (AEs), including serious and severe AEs overall, by dose level, and by severity Grade

Tolerability of CART-TnMUC1 in solid tumors and multiple myelomaUp to 2 years

Frequency of treatment emergent AEs, frequency of clinical changes from baseline in vital signs, changes in electrocardiogram, and hematology and chemistry laboratory shifts from baseline

Feasibility of CART-TnMUC1 in solid tumors and multiple myelomaUp to 2 years

Proportion of enrolled patients who did not receive CART-TnMUC1 cells

Trial Locations

Locations (10)

University of Chicago Medical Center

🇺🇸

Chicago, Illinois, United States

Hospital of the University of Pennsylvania

🇺🇸

Philadelphia, Pennsylvania, United States

University of Pittsburgh Medical Center

🇺🇸

Pittsburgh, Pennsylvania, United States

Sarah Cannon Research Institute

🇺🇸

Nashville, Tennessee, United States

The Angeles Clinic and Research Institute

🇺🇸

Los Angeles, California, United States

Mayo Clinic of Arizona

🇺🇸

Phoenix, Arizona, United States

Moffitt Cancer Center

🇺🇸

Tampa, Florida, United States

Washington University School of Medicine

🇺🇸

Saint Louis, Missouri, United States

The University of Texas MD Anderson Cancer Center

🇺🇸

Houston, Texas, United States

University of Washington Medical Center

🇺🇸

Seattle, Washington, United States

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