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Does the Reduction of Total Body Iron Storage (TBIS) Alter Mortality in a Population of Patients With Advanced PVD?

Phase 3
Completed
Conditions
Atherosclerosis
Intermittent Claudication
Peripheral Vascular Diseases
Interventions
Procedure: Ferritin reduction to 25 ng/ml by phlebotomy
Registration Number
NCT00032357
Lead Sponsor
US Department of Veterans Affairs
Brief Summary

Veterans Affairs Cooperative Study #410, The Iron and Atherosclerosis Trial, FeAST, a 24-hospital prospective randomized single-blinded clinical trial of graded iron reduction was conducted between May 1, 1999 and April 30, 2005, and has now been completed. A total of 1,277 primarily male participants with peripheral arterial disease were entered. The primary outcome was all cause mortality and the secondary outcome combined death plus non-fatal myocardial infarction (MI) and stroke.

Detailed Description

The original JAMA abstract (2007) reported no overall effect of iron reduction intervention by phlebotomy. However, pre-planned analyses according to randomization variables at entry, including age and ferritin level, were described in the JAMA paper showing improved outcomes with iron reduction with younger age by quartile for the secondary endpoint (p for interaction =0.004) and also suggested a favorable effect in smokers (p for interaction 0.006). Age analyzed as a continuous variable using the Cox proportional hazards regression model and log relative hazard plots revealed that age interacted nonlinearly with treatment in both primary (p=0.04) and secondary (p\<0.001) outcomes. The Cox model showed improved primary (HR 0.47, 95% CI 0.24-0.90, p=0.02) and secondary (HR 0.41, 95% CI 0.24-0.68, p\<0.001) outcomes in youngest age quartile participants (age 43 to 61) randomized to iron reduction versus control. Thus, an interaction between age and level of body iron may have masked beneficial effects of iron reduction in the overall cohort.

Detailed analysis of the effect of age and ferritin levels published in the American Heart Journal confirmed that iron reduction significantly improved primary and secondary outcomes in youngest age quartile participants, as described above, displayed as Kaplan-Meier plots. Mean follow-up ferritin levels (MFFL) declined with increasing entry age in controls. Older age (p=0.026) and higher ferritin (p\<0.001) at entry predicted poorer compliance with phlebotomy and rising MFFL in iron reduction participants. Iron reduction intervention also produced greater ferritin reduction in younger participants. Improved outcomes with lower MFFL occurred in iron reduction patients for both primary (HR 1.11, 95% CI 1.01-1.23, p=0.028) and secondary (HR 1.10, 95% CI 1.0-1.20, p=0.044) outcomes, and for the entire cohort: primary outcome (HR 1.11, 95% CI 1.01-1.23, p=0.037). Improved outcomes occurred with MFFL below versus above the median of the entire cohort means: primary outcome HR 1.48, 95% CI 1.14-1.92, p=0.003; secondary outcome HR 1.22, 95% CI 0.99-1.50, p=0.067.

Thus, lower iron burden predicted improved outcomes overall and was enhanced with iron reduction by phlebotomy. Controlling iron burden may improve survival, and prevent or delay non-fatal myocardial infarction and stroke. These findings warrant confirmation using further studies.

A possible effect of iron levels on risk of cancer as well as vascular disease was recognized at trial inception. Participants with visceral malignancy within the preceding five years were excluded from this study. However, information was collected prospectively on the occurrence of new visceral malignancy and cause-specific mortality including death due to cancer. As reported in the Journal of the National Cancer Institute, a new visceral malignancy was diagnosed during follow-up in 60 control and 38 iron reduction participants, a 37% (HR 0.63; 95% CI = 0.42 - 0.95, p = 0.026) decrease in risk with iron reduction. Reduced cancer risk with iron reduction was confirmed on time-to-event analysis (HR = 0.65; 95% CI = 0.43 - 0.97, p = 0.036). Reduced risk was observed for several common tumor types. Iron reduction participants had lower cancer - specific mortality and lower all-cause mortality in participants diagnosed with cancer (HR = 0.39; 95% CI = 0.21 - 0.72, p = 0.003 and HR = 0.49; 95% CI = 0.29 - 0.83, p = 0.009 respectively), compared to control participants. MFFL during follow-up in those participants randomized to iron reduction who developed cancer were comparable to levels in control participants (t93 = 0.8, p = 0.428). The MFFL in participants randomized to iron reduction developing cancer was 127 ng/mL, 95% CI = 71.2 - 183.0. The MFFL was significantly lower in participants not developing cancer, 76.4 ng/mL, 95% CI = 71.4 - 81.4, p = 0.017). Participants randomized to iron reduction developing cancer appeared to be relatively non-compliant with intervention.

Analysis of data from the FeAST study continues to delineate interactions between iron status and smoking, lipid levels and statin use, diabetes and race. It has been shown that ferritin levels ranging from about 70 to 79 ng/mL are associated with lower mortality and levels of inflammatory markers. Statin use, while not a randomization variable, has been monitored and shown to relate to lower ferritin levels.

Recruitment & Eligibility

Status
COMPLETED
Sex
All
Target Recruitment
1277
Inclusion Criteria
  1. Males over the age of 21 years and post menopausal (either natural or surgical) females with a diagnosis of intermittent claudication who are not scheduled for major surgery and who can give informed consent will be entered.
  2. Hematocrit of 30% or greater for females and 35% or greater for males, normal liver function, serum creatinine less than 4 mg/dl. Patients with mild anemia and mild creatinine elevation will be entered (provided the anemia is not due to Fe deficiency found on screening laboratory tests) because such findings are commonly present chronically in PVD.
  3. Absence of a disturbance in Fe balance (e.g. hemosiderosis from any cause, hemochromatosis, atransferrinemia, PNH, Fe deficiency)
  4. Absence for at least six months of a disease that has caused bleeding (e.g. peptic ulcer, inflammatory bowel disease, hemorrhagic diathesis )
  5. Absence of associated neoplasm other than epithelial ( non-melanoma) tumors of skin or other co-morbid condition that is expected to be fatal within one year.
  6. Absence of an associated obvious inflammatory disorder (e.g. infection, connective tis-sue disease) capable of elevating ferritin levels acutely.
  7. Patients will not be excluded on the basis of either the existence or severity of either coronary- or cerebrovascular disease, medication use including non-steroidal anti-inflammatory drugs and anticoagulants, coronary angiographic findings, previous history of or possible future need for angioplasty or coronary bypass surgery, or elevated blood pressure.
  8. Patients must agree to not take any Fe supplements or vitamins while on study.
Exclusion Criteria
  1. Patients must have at least one lower extremity and must not be on another experimental therapy protocol for atherosclerotic vascular disease.

Study & Design

Study Type
INTERVENTIONAL
Study Design
PARALLEL
Arm && Interventions
GroupInterventionDescription
Arm 1Ferritin reduction to 25 ng/ml by phlebotomyUsual care plus Ferritin reduction to a calculated nadir of 25 ng/mL by phlebotomy
Primary Outcome Measures
NameTimeMethod
MortalityThe minimum follow-up was 3.5 years and maximum follow-up was 6 years

The primary objective of this study is to evaluate the effectiveness of a reduction of Total Body Iron Stores (TBIS) in decreasing the rate of all cause mortality in patients with peripheral vascular disease (PVD).

Secondary Outcome Measures
NameTimeMethod

Trial Locations

Locations (24)

VA Sierra Nevada Health Care System

🇺🇸

Reno, Nevada, United States

VA Medical Center, Cleveland

🇺🇸

Cleveland, Ohio, United States

VA Medical Center, Birmingham

🇺🇸

Birmingham, Alabama, United States

VA Medical Center, Providence

🇺🇸

Providence, Rhode Island, United States

VA Medical Center, Long Beach

🇺🇸

Long Beach, California, United States

Central Arkansas VHS Eugene J. Towbin Healthcare Ctr, Little Rock

🇺🇸

No. Little Rock, Arkansas, United States

VA Palo Alto Health Care System

🇺🇸

Palo Alto, California, United States

Wlliam S. Middleton Memorial Veterans Hospital, Madison

🇺🇸

Madison, Wisconsin, United States

Zablocki VA Medical Center, Milwaukee

🇺🇸

Milwaukee, Wisconsin, United States

VA Stratton Medical Center, Albany

🇺🇸

Albany, New York, United States

VA Medical Center, Louisville

🇺🇸

Louisville, Kentucky, United States

Edward Hines, Jr. VA Hospital

🇺🇸

Hines, Illinois, United States

VA Medical Center, Jamaica Plain Campus

🇺🇸

Boston, Massachusetts, United States

VA Medical Center, Durham

🇺🇸

Durham, North Carolina, United States

North Florida/South Georgia Veterans Health System

🇺🇸

Gainesville, Florida, United States

Michael E. DeBakey VA Medical Center (152)

🇺🇸

Houston, Texas, United States

New York Harbor HCS

🇺🇸

New York, New York, United States

VA Pittsburgh Health Care System

🇺🇸

Pittsburgh, Pennsylvania, United States

VA Salt Lake City Health Care System, Salt Lake City

🇺🇸

Salt Lake City, Utah, United States

VA Medical Center, San Juan

🇵🇷

San Juan, Puerto Rico

VA Medical & Regional Office Center, White River

🇺🇸

White River Junction, Vermont, United States

VA Connecticut Health Care System (West Haven)

🇺🇸

West Haven, Connecticut, United States

James A. Haley Veterans Hospital, Tampa

🇺🇸

Tampa, Florida, United States

VA Medical Center, Lexington

🇺🇸

Lexington, Kentucky, United States

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