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Evaluating the Impact of Incremental Doses of a Sugar Replacer Blend on Gastrointestinal Tolerance in Chocolate

Not Applicable
Not yet recruiting
Conditions
Gastrointestinal Tolerance
Healthy Population
Interventions
Other: Milk Chocolate
Registration Number
NCT06479330
Lead Sponsor
Mondelēz International, Inc.
Brief Summary

This study is a randomized, double-blind, crossover trial aiming at evaluating the gastrointestinal tolerance of sugar replacer ingredients and blends (maltitol, kestose (Oligofructose), kestose + cocoa and polydextrose premix at different doses)

Detailed Description

The study will have a randomized, double-blind, crossover design with 5 visits consisting of one screening visit and 5 study visits (3-14-day interval between the start of each visit) across 2-8 weeks. Participants will be randomized to a test sequence and will consume one control product and 4 test products made with the sugar replacer ingredients or blend of ingredients over the course of the study. At each visit, eligible participants will come to the lab between 8-11am, and \~1-2 hours after consuming their usual breakfast at home. After rating the severity of gastrointestinal symptoms, participants will consume one of the investigational products with a drink of water (250ml) and then be free to leave. The severity of 8 gastrointestinal symptoms (abdominal bloating, abdominal pain, flatulence, burping, reflux (heartburn), stomach rumbling (borborygmus), nausea and vomiting) will be rated at 2, 4, 6, 10 and 24 hours after starting to eat. Over the 24 hour period following consuming the investigational product a Bowel Habit Diary will be kept. For each bowel movement passed during the 24 hour period, participants will be asked to record: the time, if they had to strain, if they experienced discomfort, if they felt there was incomplete evacuation and the consistency of the stool rated using the Bristol Stool Scale (BSS).

Recruitment & Eligibility

Status
NOT_YET_RECRUITING
Sex
All
Target Recruitment
60
Inclusion Criteria
  • Non-pregnant, non-lactating, healthy individuals aged 18-55 years, inclusive
  • Body Mass Index (BMI) 18.5-32.0kg/m², inclusive
  • No major illness, trauma or surgery requiring hospitalization within 3 months of the screening visit
  • Ability to understand the study procedures and willing to provide informed consent to participate in the study
  • Non-smokers or smokers who smoke <10 cigarettes/day and are willing not to change nicotine habits during the study period
  • Willing to limit alcohol consumption to ≤3 standard drinks/day and ≤7 standard drinks/week during the study period
  • Willing to refrain from any marijuana or hemp products during the study period
  • Normal bowel habits (>2 bowel movements/week and <3 bowel movements per day)
  • Consumes ≤4 servings/day of fruits and vegetables combined and ≤3 (women) or ≤4 (men) servings/day of whole grains using the definitions of "serving" .
  • Participants must have a cell phone/tablet/computer and be willing and able to use it to collect study data
  • Participants must be eligible to receive income in Canada and be covered by a health insurance plan such as OHIP (Ontario Health Insurance Plan)
  • Participants are willing to follow current COVID guidelines with respect to attending study visits
Exclusion Criteria
  • Failure to meet any one of the inclusion criteria
  • Chronic moderate to severe gastrointestinal symptoms
  • Use of systemic antibiotics, antifungals or antiparasitics in the past 3 months and during the experimental period
  • Use of medications, supplements, and products which may affect the results (laxative, anti-diarrhea, anti-constipation drugs, high fiber supplements)
  • Individuals with any medical conditions or use of supplements or medications that increase risk to the subject or others or may affect the results, as judged by the Principal - Investigator
  • Unwillingness or inability to comply with the experimental procedures and to follow INQUIS safety guidelines
  • Known intolerance, sensitivity, or allergy to any ingredients in the study test products
  • Subject is currently participating or recently (within 30 days of screening) participated in a clinical trial involving long-term exposure (greater than 24 hours) to an investigational drug, nutritional supplement, or lifestyle modification

Study & Design

Study Type
INTERVENTIONAL
Study Design
CROSSOVER
Arm && Interventions
GroupInterventionDescription
Kestose + cocoa and polydextrose premix dose 2 chocolateMilk ChocolateMillk chocolate with kestose oligofructose + cocoa and polydextrose premix dose 2 replacing all sugar at a dose of 45 g (2 servings)
Maltitol chocolateMilk ChocolateMilk chocolate with maltitol replacing all sugar at a dose of 45 g (2 servings)
Control chocolate with sugarMilk ChocolateMilk chocolate made with sugar at a dose of 45 g (2 servings)
Kestose chocolateMilk ChocolateMilk chocolate with kestose oligofructose replacing all sugar at a dose of 45 g (2 servings)
Kestose + cocoa and polydextrose premix dose 1 chocolateMilk ChocolateMillk chocolate with kestose oligofructose + cocoa and polydextrose premix dose 1 replacing all sugar at a dose of 45 g (2 servings)
Primary Outcome Measures
NameTimeMethod
Total Area Under the Curve (tAUC) of composite gastrointestinal Symptom scoretAUC between 0 and 24 hours

total area under the curve (tAUC) of the composite GastroIntestinal symptom score (sum of the scores for the 8 GI symptoms). The score values can range from 0 up to 24 with the higher values showing the higher levels of symptoms (worse conditions).

Secondary Outcome Measures
NameTimeMethod
Frequency of Diarrhea within 24 hoursEvaluation performed over 24 hour after consumption of study products

Proportion of participants with diarrhea, defined as 3 or more type 6 or 7 stools (based on the BSS) within the 24 hour period after consumption of the study products

Maximum score for burpingEvaluation performed at time 0, 2, 4, 6, 10 and 24 hours

Maximum detected score (which can range from 0 up to 3)

Maximum score for refluxEvaluation performed at time 0, 2, 4, 6, 10 and 24 hours

Maximum detected score (which can range from 0 up to 3)

Time for maximum score for refluxEvaluation performed at time 0, 2, 4, 6, 10 and 24 hours

Time of the maximum score

Maximum score for stomach rumblingEvaluation performed at time 0, 2, 4, 6, 10 and 24 hours

Maximum detected score (which can range from 0 up to 3)

Time for maximum score for abdominal bloatingEvaluation performed at time 0, 2, 4, 6, 10 and 24 hours

Time of the maximum score

Maximum score for flatulenceEvaluation performed at time 0, 2, 4, 6, 10 and 24 hours

Maximum detected score (which can range from 0 up to 3)

Time of maximum score for abdominal painEvaluation performed at time 0, 2, 4, 6, 10 and 24 hours

Time of the maximum score

Maximum score for nauseaEvaluation performed at time 0, 2, 4, 6, 10 and 24 hours

Maximum detected score (which can range from 0 up to 3)

Maximum score for vomitingEvaluation performed at time 0, 2, 4, 6, 10 and 24 hours

Maximum detected score (which can range from 0 up to 3)

Time for maximum score for vomitingEvaluation performed at time 0, 2, 4, 6, 10 and 24 hours

Time of the maximum score

incomplete evacuationEvaluation performed over 24 hour after consumption of study products

Proportion of participants declaring incomplete evacuation or not

Stool consistency based on bristol stool scaleEvaluation performed over 24 hour after consumption of study products

mean stool consistency on Bristol Stool Scale (BSS). The scale provide values from 1 to 7 depending on the type of stool with 1 being the driest stools and 7 the most liquid one.

Total Area Under the Curve of each individual gastrointestinal symptomtAUC between 0 and 24 hours

Total Area Under the Curve of each of the individual 8 gastrointestinal symptoms (abdominal bloating, abdominal pain, flatulence, burping, reflux, stomach rumbling, nausea, vomiting). Each symptom range from 0 up to 3 with 3 showing the highest / worse condiiton of symptoms

Maximum score for abdominal bloatingEvaluation performed at time 0, 2, 4, 6, 10 and 24 hours

Maximum detected score (which can range from 0 up to 3)

Maximum score for abdominal painEvaluation performed at time 0, 2, 4, 6, 10 and 24 hours

Maximum detected score (which can range from 0 up to 3)

Time for maximum score for flatulenceEvaluation performed at time 0, 2, 4, 6, 10 and 24 hours

Time of the maximum score

Frequency of composite score > 1 at each time pointEvaluation performed at time 0, 2, 4, 6, 10 and 24 hours

Proportion of participants who have a composite GastoInestinal symptom score \>1 (moderate-to-severe) at each time point. The composite score can range from 0 up to 24 with the higher values showing the higher levels of symptoms (worse conditions)

Time for maximum score for burpingEvaluation performed at time 0, 2, 4, 6, 10 and 24 hours

Time of the maximum score

Number of bowel movementsEvaluation performed over 24 hour after consumption of study products

Number of bowel movements over the 24 hours

discomfort during bowel movementEvaluation performed over 24 hour after consumption of study products

Proportion of participants having discomfort or not

Time for maximum score for stomach rumblingEvaluation performed at time 0, 2, 4, 6, 10 and 24 hours

Time of the maximum score

Time for maximum score for nauseaEvaluation performed at time 0, 2, 4, 6, 10 and 24 hours

Time of the maximum score

straining during bowel movementEvaluation performed over 24 hour after consumption of study products

Proportion of participants having to strain or not

Trial Locations

Locations (1)

Inquis Clinical Research

🇨🇦

Toronto, Ontario, Canada

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