Ascitic Fluid Microbiome in Liver Cirrhosis

Withdrawn

• Conditions: Polymerase Chain Reaction; Microbiota; Ascitic Fluid, Microbiota; Sequence Analysis
• Interventions: Other: Ascitic fluid puncture; Other: Skin swaps from puncture side; Other: Stool samples

• Registration Number: NCT02813590
• Lead Sponsor: Medical University of Graz

Brief Summary

Ascitic fluid microbiome is going to be investigated in this study by using internal transcripted spacer (ITS) and 16S ribosomal RNA (rRNA) polymerase chain reaction (PCR) and sequencing as conventional culture methods are lacking sensitivity regarding diagnosis of spontaneous bacterial peritonitis (SBP).

All patients with routinely performed ascitic fluid puncture and underlying liver cirrhosis are included in this study. Next generation sequencing (NGS) in ascitic fluid, skin swaps from puncture side and stool samples as well as conventional culture methods are performed to investigate the difference in the microbiome between patients with and without SBP.

Detailed Description

Not available

Study Timeline

• Study Start: 2016-06
• Study First Submitted: 2016-06-16
• Study First Submitted QC: 2016-06-22
• Study First Posted: 2016-06-27
• Primary Completion: 2017-09
• Study Completion: 2017-09
• Status Verified: 2024-03
• Last Update Submitted: 2024-03-12
• Last Update Posted: 2024-03-15

Recruitment & Eligibility

• Status: WITHDRAWN
• Sex: All
• Target Recruitment: Not specified

Inclusion Criteria

• Underlying decompensated liver cirrhosis
• routinely performed ascitic fluid puncture
• informed consent

Exclusion Criteria

Study & Design

• Study Type: OBSERVATIONAL
• Study Design: Not specified

Arm && Interventions

Group	Intervention	Description
Patients with liver cirrhosis and with SBP	Stool samples	-
Patients with liver cirrhosis and with SBP	Skin swaps from puncture side	-
Patients with liver cirrhosis and without SBP	Ascitic fluid puncture	-
Patients with liver cirrhosis and without SBP	Skin swaps from puncture side	-
Patients with liver cirrhosis and with SBP	Ascitic fluid puncture	-
Patients with liver cirrhosis and without SBP	Stool samples	-

Primary Outcome Measures

Name	Time	Method
Next generation sequencing results from ascitic fluid samples	1 year

Trial Locations

Locations (1)

Medical University of Graz; 🇦🇹 Graz, Styria, Austria