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Safety Study of Natalizumab to Treat Multiple Sclerosis (MS)

Phase 1
Completed
Conditions
Relapsing-Remitting Multiple Sclerosis
Secondary Progressive Multiple Sclerosis
Interventions
Other: standard of care
Registration Number
NCT00559702
Lead Sponsor
Biogen
Brief Summary

The primary objective of this study is to compare the pharmacokinetic (PK) and pharmacodynamics (PD) of single subcutaneous (SC) and intramuscular (IM) doses of 300 mg natalizumab to intravenous (IV) administration of 300 mg natalizumab in multiple sclerosis (MS) participants. The secondary objectives are to investigate the safety, tolerability and PK of repeated natalizumab doses administered SC and IM, to investigate the immunogenicity of repeated natalizumab doses administered SC and IM, to explore proof of concept within the secondary progressive multiple sclerosis (SPMS) population using change from baseline in clinical measures including: expanded disability status scale (EDSS), multiple sclerosis functional composite scale (MSFC), symbol digit modalities test (SDMT), visual analogue scale (VAS), and visual function test; and brain magnetic resonance imaging (MRI) measures including: number of new or newly-enlarging T2 hyperintense lesions, number of new T1 hypointense lesions, number of new gadolinium-enhancing (Gd+) lesions, whole brain atrophy, magnetization transfer ratio (MTR), and diffusion tensor imaging (DTI) and to observe the effect of natalizumab administered IV and SC on brain MRI measures in participants with relapsing forms of MS.

Detailed Description

Not available

Recruitment & Eligibility

Status
COMPLETED
Sex
All
Target Recruitment
76
Inclusion Criteria
  • For arms 1,2,3 and 4: Diagnosis of Secondary Progressive Multiple Sclerosis (SPMS)
  • For arms 5 and 6: Diagnosis of relapsing forms of Multiple Sclerosis (MS).
  • No past history of receiving natalizumab.

Key

Exclusion Criteria
  • For arms 1,2,3 and 4 Diagnosis of primary progressive MS or relapsing-remitting MS.
  • Form arms 5 and 6: Diagnosis of primary progressive MS or secondary progressive MS without the occurrence of relapses.

NOTE: Other protocol defined inclusion/exclusion criteria may apply.

Study & Design

Study Type
INTERVENTIONAL
Study Design
PARALLEL
Arm && Interventions
GroupInterventionDescription
2natalizumabNatalizumab IM (Participants with secondary progressive multiple sclerosis)
1natalizumabNatalizumab IV (Participants with secondary progressive multiple sclerosis)
4standard of careStandard of care as determined by the Investigator and Treating Neurologist (Participants with secondary progressive multiple sclerosis)
3natalizumabNatalizumab SC (Participants with secondary progressive multiple sclerosis)
6natalizumabNatalizumab IV (Participants with relapsing forms of multiple sclerosis)
5natalizumabNatalizumab SC (Participants with relapsing forms of multiple sclerosis)
Primary Outcome Measures
NameTimeMethod
Maximum observed concentration (Cmax) of natalizumabPre-dose, 4, 24, 48, 72 and 96 hours post-dose and Days 7, 14, 21, 28, 35, 42 and 56
Time to maximum observed concentration (Tmax) of natalizumabPre-dose, 4, 24, 48, 72 and 96 hours post-dose and Days 7, 14, 21, 28, 35, 42 and 56
Area under the curve to the last measurable concentration (AUC0-last) of natalizumabPre-dose, 4, 24, 48, 72 and 96 hours post-dose and Days 7, 14, 21, 28, 35, 42 and 56

Area under the curve to the last measurable concentration as measured by the trapezoidal rule.

Apparent volume of distribution of natalizumabPre-dose, 4, 24, 48, 72 and 96 hours post-dose and Days 7, 14, 21, 28, 35, 42 and 56
Half-life of natalizumabPre-dose, 4, 24, 48, 72 and 96 hours post-dose and Days 7, 14, 21, 28, 35, 42 and 56
Area under the curve extrapolated to infinity (AUC0-∞) of natalizumabPre-dose, 4, 24, 48, 72 and 96 hours post-dose and Days 7, 14, 21, 28, 35, 42 and 56
Apparent Clearance of natalizumabPre-dose, 4, 24, 48, 72 and 96 hours post-dose and Days 7, 14, 21, 28, 35, 42 and 56
α4-integrin saturationPre-dose, 4, 24 and 72 hours post-dose and Days 7, 14, 21, 28, 35, 42 and 56

PD activity will be assessed by measuring the degree of natalizumab saturation of the very late antigen-4 (also known as α4β1 integrin) VLA-4 (α4β1) receptor on peripheral blood lymphocyte/monocyte populations.

Secondary Outcome Measures
NameTimeMethod
Number of Participants with adverse events13-19 months
Number of participants with abnormalities in vital signs13-19 months
Number of participants with changes in the physical examination13-19 months
Number of participants with abnormal laboratory test results13-19 months
Number of participants with natalizumab antibodiesDays 28, 42, 56, Weeks 24 and 32
Change from Baseline in expanded disability status scale (EDSS)Baseline, Weeks 8, 20, and 32

The EDSS measures disability status on a scale ranging from 0 to 10, with higher scores indicating more disability. Scoring is based on measures of impairment in eight functional systems on examination by a neurologist.

Change form Baseline in Multiple Sclerosis Functional Composite Scale (MFSC)Baseline, Weeks 8, 20, and 32

The MFSC consists of 3 tests: 1. Timed 25-Foot Walk, a quantitative mobility and leg function performance test where the participant is timed while walking for 25 feet; 2. 9-Hole Peg Test (9HPT), a quantitative test of upper extremity function that measures the time it takes to place 9 pegs into 9 holes and then remove the pegs. 3. 3 Second Paced Auditory Serial Addition Test (PASAT 3). The MSFC is based on the concept that scores for these 3 dimensions - arm, leg, and cognitive function are combined to create a single score that can be used to detect change over time. A composite z-score is created, which represents the number of standard deviations (SDs) a participant's test result is higher (z \> 0) or lower (z \< 0) than the average test result (z = 0) of the reference population.

Change from Baseline in Symbol Digit Modalities Test (SDMT)Baseline, Weeks 8, 20, and 32

SDMT is a screening test for cognitive impairment. Participants are given 90 seconds in which to pair specific numbers with given geometric figures using a key. Scores range from 0 to 110 (best).

Change from Baseline in visual analog scale (VAS)Baseline, Weeks 8, 20, and 32

The participant's global assessment of well-being as assessed using a visual analogue scale (VAS) is a quality of life measurement that will be evaluated for the specified time periods. Participants report how they feel on a scale of 0 to 100, where 0 indicates being "poor" and 100 being "excellent."

Change from Baseline in visual function testBaseline, Weeks 8, 20, and 32
Number of new or newly enlarging T2 hyperintense lesionsBaseline and Week 32

Measured by magnetic resonance imaging (MRI).

Number of new gadolinium-enhanced lesionsBaseline and Week 32

Measured by magnetic resonance imaging (MRI).

Number of new T1 hypointense lesionsBaseline and Week 32

Measured by magnetic resonance imaging (MRI).

Whole brain atrophyBaseline and Week 32

Atrophy will be measured as the percent brain volume change (PBVC) and will be assessed using the Structural Image Evaluation of Normalized Atrophy (SIENA).

Percent change in magnetization transfer ratio (MTR)Baseline and Week 32

Remyelination will be measured using magnetization transfer ratio (MTR) in whole brain (WB) and normal-appearing brain tissue (NABT),

Diffusion tensor imaging (DTI)Baseline and Week 32
Injection site pain assessmentPre-dose, 5 and 15 minutes and 24 hours post-dose

Trial Locations

Locations (1)

Research Site

🇺🇸

Vienna, Virginia, United States

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