Screening and Identification of Abnormal Expression Proteins and miRNA From Colonic Mucosa of Patients With Irritable Bowel Syndrome.

Chinese PLA General Hospital0 sites180 target enrollmentJanuary 2006

ConditionsIrritable Bowel Syndrome Digestive System Diseases Gastrointestinal Diseases Intestinal Diseases Colonic Diseases Colonic Diseases, Functional

Overview

Phase: Not Applicable
Intervention: Not specified
Conditions: Irritable Bowel Syndrome
Sponsor: Chinese PLA General Hospital
Enrollment: 180
Last Updated: 15 years ago

Overview Investigators Eligibility Criteria Outcomes Similar Trials

Overview

Brief Summary

In the investigators study, the investigators will focus on the screening of the related proteins and miRNA to IBS in order to reveal the possible clues or molecular mechanism for this disorder.

Detailed Description

For the proteomic procedures, Our study groups extracted proteins from the cecum and sigmoid colon samples and ran on the two-dimensional gel electrophoresis (2-DE). The proteins on 2-DE were visualized by the silver stain, and then gel maps were scanned and analyzed with the Image Master 2D Elite. The obvious abnormal protein spots were identified by the mass spectrometry. Western blot 、 immunohistochemistry 、 mRNA and miRNA were also performed for further verification of the proteins.

Registry

clinicaltrials.gov

Start Date

January 2006

End Date

December 2012

Last Updated

15 years ago

Study Type

Observational

Sex

All