Association Between Mitochondrial DNA Copy Number in Peripheral Blood Cells and Risk of Developing Coronary Heart Disease
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 发起方
- 入组人数
- 400
- 试验地点
- 1
- 主要终点
- Relative copy number of mitochondrial DNA
研究概览
简要总结
Compelling epidemiological evidence indicates that alterations of mitochondrial DNA, including mutations and abnormal content of mitochondrial DNA (mtDNA), are associated with the initiation and development of cardiovascular disease. This study was undertaken to investigate whether mtDNA copy number in peripheral blood cells could be used as a risk predictor for coronary heart disease.
研究设计
- 研究类型
- Observational
- 观察模型
- Case Control
- 时间视角
- Retrospective
入排标准
- 年龄范围
- 18 Years 至 85 Years（Adult, Older Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •History of documented myocardial infarction;
- •Prior coronary revascularization intervention (coronary artery bypass graft surgery or percutaneous coronary intervention);
- •The presence of ≥ 50% stenosis in one or more coronary arteries identified during cardiac catheterization;
排除标准
- •History of malignancy or end-stage renal disease;
- •Blood transfusion within one month or prior bone marrow transplantation
- •Patients who reluctant to sign informed consent
结局指标
主要结局
Relative copy number of mitochondrial DNA
时间窗: From date of percutaneous coronary intervention until the date of discharging from hospital, assessed up to 5 days
The ratio of mitochondrial DNA copy number to hemoglobin copy number was calculated for each sample from standard curves. After that, the ratio for each sample was normalized to a calibrator DNA in order to standardize between different runs, and then defined as the measurement of relative mtDNA copy number.Relative copy number of Mitochondrial DNA were measured in CHD patients in comparison to control group.
次要结局
未报告次要终点
研究者
Wenyi Guo
Deputy Director of Department of Cardiovascular Medicine
Air Force Military Medical University, China