Intravenous Stem Cells After Ischemic Stroke

Phase 2

Completed

• Conditions: Ischemia; Stroke
• Interventions: Genetic: Autologous mesenchymal stem cells

• Registration Number: NCT00875654
• Lead Sponsor: University Hospital, Grenoble

Brief Summary

The main objective of the study is to evaluate feasibility and tolerance of the intravenous injection of autologous mesenchymal stem cells for patients presenting an ischemic stroke (less than 6 weeks).

Detailed Description

Stroke is the leading cause of acquired adult disability. Except the hospitalization in stroke units, only thrombolysis has been shown to be efficient to treat acute ischemic stroke in the first three hours after the onset. Increasing brain plasticity after stroke represents an important alternative strategy. Cell therapy provides a functional improvement after cerebral ischemia in rodent models. This "restorative" therapy aims to replace destroyed cerebral tissue with a stem cells graft. Despite these encouraging experiments, we do not know yet the best way of administration of the stem cells, the best dose and the optimal delay of the graft. The pioneer clinical studies failed to reproduce this benefit for patients probably because of the limited number of studied patients. Therefore, more translational studies are needed to improve our knowledge in this promising field. Among different cell sources, mesenchymal (or stromal) stem cells (MSC) derived from bone marrow offer the advantage of arising from a non tumoral and no modified source and are not sources of immunological or ethical problems.

Our research project involves a development of cell therapy in a phase IIa clinical trial of feasibility and safety in patients (randomised, controlled, open, with 3 parallel groups).

Study Timeline

• Study First Submitted: 2009-04-02
• Study First Submitted QC: 2009-04-02
• Study First Posted: 2009-04-03
• Study Start: 2010-08
• Status Verified: 2017-10
• Primary Completion: 2017-10-20
• Study Completion: 2017-10-20
• Last Update Submitted: 2017-10-30
• Last Update Posted: 2017-11-01

Recruitment & Eligibility

• Status: COMPLETED
• Sex: All
• Target Recruitment: 31

Inclusion Criteria

• right or left carotid ischemic stroke in the 14 previous days, confirmed by MRI.
• Persistent neurological deficit (NIHSS >=7).
• Optimal medical treatment(antithrombotics, antihypertensive, statins).
• General state compatible with a program of functional rehabilitation.

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Exclusion Criteria

• Severe extensive stroke implying vital prognosis.
• Severe persistent neurological deficit (NIHSS > 24).
• Medical history of neurological pathology with a deficit as consequence (Rankin < 3 before stroke).
• Serious psychiatric disease.
• Myocardial infarction less than 3 month old.
• Recurring thromboembolic disease or less than 6 month old.
• Patient with organ transplantation.
• Medical history of infection (HIV,HTLV, HBV, HCV).
• Current immunosuppressive/immunomodulating treatment.
• Medical history of cancer.
• Medical history of chemotherapy.
• Known chronic kidney failure(clearance of creatinin < 90 ml/min/1,73m2).
• Known hepatic failure(diminution of prothrombin level (TP) not corrigiable with vitamin K).
• Obesity hinding the bone-marrow sampling in the iliac crest.
• Pathology implying vital prognosis in the 3 month following stroke.
• Refusal to participate.
• Patient unable to give personally his/her consent.
• Pregnant, parturient and feeding women.
• Woman in age to procreate who could not receive an effective method of contraception during the study.
• Participation to another therapeutic clinical trial or in period of exclusion of a therapeutic clinical study.
• Privation of liberty with a decision of justice or administration, legal protection.
• Non affiliation to social security.

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Study & Design

• Study Type: INTERVENTIONAL
• Study Design: PARALLEL

Arm && Interventions

Group	Intervention	Description
2	Autologous mesenchymal stem cells	First dose of stem cells
3	Autologous mesenchymal stem cells	Second dose of stem cells

Primary Outcome Measures

Name	Time	Method
feasibility and tolerance of the intravenous injection of autologous mesenchymal stem cells in patients with carotid ischemic stroke	2 weeks, 1, 2, 4, 6 months and 1, 2 years

Secondary Outcome Measures

Name	Time	Method
Determination of the most effective dose of stem cells	2 weeks, 1, 2, 4, 6 months and 1, 2 years
To define the best target population for a future study	2 weeks, 1, 2, 4, 6 months and 1, 2 years
To define the best criteria for a future trial (phase III)	2 weeks, 1, 2, 4, 6 months and 1, 2 years
Clinical and functional effects of the intravenous injection of autologous mesenchymal stem cells in patients with carotid ischemic stroke	2 weeks, 1, 2, 4, 6 months and 1, 2 years

Trial Locations

Locations (3)

Neuroradiology/MRI, University Hospital of Grenoble; 🇫🇷 Grenoble, France

Stroke Unit, University Hospital of Grenoble; 🇫🇷 Grenoble, France

Tissular and cell therapy unit, UniversityHospitalof Grenoble; 🇫🇷 Grenoble, France