Glycocalyx Damage During Normothermic Graft Perfusion in Orthotopic Liver Transplantation - A Pilot Study
Trial Snapshot
- Phase
- Not Applicable
- Status
- Completed
- Sponsor
- Enrollment
- 30
- Locations
- 2
- Primary Endpoint
- Syndecan 1 level during normothermic machine perfusion
Study Overview
Brief Summary
An organ intended for transplantation is normally stored on ice (cold storage, CS) after explantation from the donor. During this storage process, damage to the endothelial glycocalyx occurs. It is known from numerous studies that the integrity of the endothelial glycocalyx is necessary for organ function. Normothermic machine perfusion is an alternative storage method for explanted livers, where the graft is perfused with warm blood. This study aims to clarify the influence of normothermic machine perfusion on endothelial glycocalyx damage of the graft.
Study Design
- Study Type
- Interventional
- Allocation
- Na
- Intervention Model
- Single Group
- Primary Purpose
- Basic Science
- Masking
- None
Eligibility Criteria
- Ages
- 18 Years to — (Adult, Older Adult)
- Sex
- All
- Accepts Healthy Volunteers
- No
Inclusion Criteria
- •First deceased donor liver transplantation receiving a graft after normothermic machine perfusion
- •Recipient age >18 years
- •Written informed consent
Exclusion Criteria
- •Age < 18 years
Arms & Interventions
Normothermic Machine Perfusion
Blood sampling at 5 time points after transplantation Perfusate collection at 3 time points during normothermic machine perfusion prior to transplantation
Intervention: Heparansulfate and Syndecan 1 (Diagnostic Test)
Outcomes
Primary Outcomes
Syndecan 1 level during normothermic machine perfusion
Time Frame: 24 hours
Syndecan 1 level at the end of normothermic perfusion compared to the beginning of perfusion
Heparansulfate level during normothermic machine perfusion
Time Frame: 24 hours
Heparansulfate level at the end of normothermic perfusion compared to the beginning of perfusion
Secondary Outcomes
- Syndecan 1 after transplantation(72 hours)
- Heparansulfate level after transplantation(72 hours)