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The Effects of Strawberries on Blood Cholesterol.

Not Applicable
Completed
Conditions
Hyper-LDL-cholesterolemia
Metabolic Syndrome
Interventions
Other: Control powder
Dietary Supplement: strawberries
Registration Number
NCT03441620
Lead Sponsor
University of Nevada, Las Vegas
Brief Summary

In this study, we propose to investigate the effects of dietary achievable doses of strawberries on serum LDL-cholesterol (LDL-C) and related lipid profiles, measures of glycemia and insulin resistance, and biomarkers of inflammation in a 14 week controlled crossover study.

Detailed Description

The screen visit will involve an 8 hour fasting state and the following procedures:

* Completing screening questionnaire

* Measuring height, weight, blood pressure, and waist size

* Drawing about 30mL blood for blood glucose and lipids and comprehensive metabolic panel All subjects will be asked to follow usual diet and lifestyle, and refrain from other sources of berries and related products while on the study. Subjects will also maintain 3-day food records at screen, 4, 9, and 14 weeks of the study. Height, weight, blood pressure and waist circumference will be measured by trained personnel at MPE 326 (KNS facility for clinical research). Blood draws will be performed by trained phlebotomists (to be hired) at MPE 326, and all procedures will be performed by trained research personnel \[PI \&Co-PI: Basu, Izuora and graduate student\]. In case of blood pressure, an average of at least three readings, 10 min apart, will be measured at each visit for each participant. Blood glucose (fasting and postprandial at two hours) will be determined at each time point. The oral glucose tolerance tests will be conducted at MPE 326 and blood samples will be sent to Quest Diagnostics, Las Vegas. Insulin resistance will be calculated using the homoeostatic model assessment (HOMA-IR). The HOMA-IR is a standard and widely used formula in calculating insulin resistance based on fasting glucose values. Screening and follow-up tests will include clinical laboratory tests for blood glucose, lipids, NMR profiles, C-reactive protein and metabolic panel to determine effects of the intervention.

Recruitment & Eligibility

Status
COMPLETED
Sex
All
Target Recruitment
33
Inclusion Criteria
  • elevated total and LDL cholesterol (>116 mg/dL)
  • body mass index (> or = 30 kg/m2)
  • features of the metabolic syndrome
Exclusion Criteria
  • taking glucose and lipid lowering medications (e.g. statins, metformin)
  • history of chronic conditions (diabetes, CHD, anemia, renal diseases)
  • allergic to strawberries
  • pregnant and/or lactating
  • smoking
  • vegetarian or consuming special diet

Study & Design

Study Type
INTERVENTIONAL
Study Design
CROSSOVER
Arm && Interventions
GroupInterventionDescription
ControlControl powderCalorie and fiber-matched control powder
Strawberry one servingstrawberriesFreeze-dried powder equivalent to one serving fresh strawberries per day.
Strawberry two-half servingsstrawberriesFreeze-dried powder equivalent to 2.5 serving fresh strawberries per day.
Primary Outcome Measures
NameTimeMethod
Serum Lipid profiles14 weeks

Serum LDL, total and HDL cholesterol, triglycerides

Serum lipid particle concentrations14 weeks

Molar concentrations of serum lipids and particle size

Glycemic control14 weeks

Blood glucose

Diabetes control14 weeks

Insulin resistance

Secondary Outcome Measures
NameTimeMethod
Systemic Inflammation14 weeks

Serum C-reactive protein and adipokines

Serum metabolomics14 weeks

primary and lipid metabolites in serum

Systemic Anthocyanins14 weeks

Strawberry anthocyanin metabolites measured in serum

Trial Locations

Locations (3)

University of Oklahoma Health Sciences Center, OCTSI

🇺🇸

Oklahoma City, Oklahoma, United States

Oklahoma State University

🇺🇸

Stillwater, Oklahoma, United States

University of Nevada at Las Vegas

🇺🇸

Las Vegas, Nevada, United States

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