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Immunoinflammatory Regulation of Esketamine in Septic Patients

Phase 4
Recruiting
Conditions
Immunosuppression
Sepsis
Inflammatory Response
Esketamine
Interventions
Registration Number
NCT04843982
Lead Sponsor
Wuhan Union Hospital, China
Brief Summary

Studies have shown that excessive systemic inflammatory response and concomitant immunosuppression are the main cause of early death in patients with sepsis. Therefore, it is very important to reduce excessive inflammation and improve immunosuppression in the acute phase of sepsis. Clinical studies have shown that esketamine combined with propofol for sedation has been proven to be safe and effective for septic patients in the ICU due to its cardiovascular stability. Previous studies have demonstrated that esketamine has anti-inflammatory effects against depression and surgical stress. Our preliminary experimental studies have found that esketamine had strong anti-inflammatory effects in the acute phase of sepsis. However, it is not clear whether esketamine could reduce excessive inflammation and improve immunosuppression in septic patients primarily sedated with a continuous infusion of propofol.

This intervention study is to investigate whether three consecutive days of intravenous esketamine infusions via infusion pump (0.07 mg/kg/h) could reduce excessive inflammation and improve immunosuppression in septic patients requiring mechanical ventilation in the ICU under sedation primarily with propofol.

Detailed Description

Not available

Recruitment & Eligibility

Status
RECRUITING
Sex
All
Target Recruitment
100
Inclusion Criteria
  • 18 years old ≤ age ≤60 years old;
  • SOFA score ≥2;
  • Mechanical ventilation should be required for at least 24 hours when included in the study;
  • Informed consent is obtained.
Exclusion Criteria
  • Age < 18 years old or ≥ 60 years old;
  • Previous solid organ or bone marrow transplantation;
  • Autoimmune diseases (rheumatoid arthritis, systemic lupus erythematosus, etc.), or hematologic malignancies (leukemia and lymphoma, etc.);
  • Received radiotherapy or chemotherapy within the past 30 days, or received immunosuppressant drugs (tripterygium wilfordii, mycophenolate mofetil, cyclophosphamide, FK506, etc.), or continuous treatment with prednisolone more than 10 mg/day (or equivalent doses of the other hormones);
  • Unstable angina pectoris or myocardial infarction in the past six months;
  • Acute brain injury (traumatic brain injury, subarachnoid hemorrhage, acute ischemic stroke, acute intracranial hemorrhage, acute intracranial infection, etc.);
  • Poorly controlled hypertension and congestive heart failure;
  • Increased intraocular or intracranial pressure;
  • Chronic kidney disease, received continuous renal replacement therapy in the past 30 days, or acute renal failure requiring CRRT;
  • Severe chronic liver disease (Child-Pugh class B or C);
  • Alcohol dependence, mental illness or severe cognitive impairment;
  • Pregnancy or lactation;
  • Informed consent is not obtained.

Study & Design

Study Type
INTERVENTIONAL
Study Design
PARALLEL
Arm && Interventions
GroupInterventionDescription
esketamine plus propofolEsketamine hydrochlorideAfter inclusion, patients are sedated primarily with propofol (0-3 mg/kg/h) using a microinfusion pump and adjusted according to the depth of sedation (Richmond Agitation Sedation Scale (RASS): 0 to -2). After inclusion, septic patients will be received a single intravenous injection of esketamine (0.7 mg/kg), and then followed by an intravenous administration of esketamine (0.07 mg/kg/h) with an infusion pump for three consecutive days.
Primary Outcome Measures
NameTimeMethod
ICU length of stayup to 8 weeks

Length of stay in the ICU

Serum concentration of inflammatory cytokines (0 h)0 hour after study inclusion

Interleukin (IL)-6, tumor necrosis factor (TNF)-α, IL-2, IL-4, IL-10, IL-17A, and interferon (IFN)-γ

Serum concentration of inflammatory cytokines (72 h)72 hours after study inclusion

IL-6, TNF-α, IL-2, IL-4, IL-10, IL-17A, and IFN-γ

Absolute number of lymphocyte subsets in the peripheral blood (0 h)0 hour after study inclusion

CD3(+), CD3(+) CD4(+), CD3(+) CD8(+), CD3(-) CD16(+) CD56(+) , and CD19(+) cells

Serum concentration of inflammatory cytokines (48 h)48 hours after study inclusion

IL-6, TNF-α, IL-2, IL-4, IL-10, IL-17A, and IFN-γ

Absolute number of lymphocyte subsets in the peripheral blood (48 h)48 hours after study inclusion

CD3(+), CD3(+) CD4(+), CD3(+) CD8(+), CD3(-) CD16(+) CD56(+) , and CD19(+) cells

Absolute number of lymphocyte subsets in the peripheral blood (72 h)72 hours after study inclusion

CD3(+), CD3(+) CD4(+), CD3(+) CD8(+), CD3(-) CD16(+) CD56(+) , and CD19(+) cells

Secondary Outcome Measures
NameTimeMethod
Acute physiology and chronic health evaluation (APACHE) Ⅱ score72 hours after study inclusion

0-67, higher scores correspond to more severe disease and a higher risk of death

Serum concentration of atrial natriuretic peptide (ANP) (48h)48 hours after study inclusion

ANP is secreted primarily by atrial cardiomyocytes

Infection complicationsThrough study completion, an average of 2 year

Pulmonary infection, urinary tract infection, bloodstream infections, etc

Serum concentration of atrial natriuretic peptide (ANP) (0 h)0 hour after study inclusion

ANP is secreted primarily by atrial cardiomyocytes

Total hospital length of stayThrough study completion, an average of 2 year

Total length of hospital stay

In-hospital mortalityThrough study completion, an average of 2 year

Mortality rates for the entire period of hospitalization

CCL1 expression in alveolar macrophages (0 h)0 hour after study inclusion

CCL1 expression in alveolar macrophages collected from bronchoalveolar lavage fluid

CCL1 expression in alveolar macrophages (24 h)24 hours after study inclusion

CCL1 expression in alveolar macrophages collected from bronchoalveolar lavage fluid

expression of WNT pathway proteins in alveolar macrophages (24 h)24 hours after study inclusion

Wnt5a, FZD5, b-catenin, Lef1 expression in alveolar macrophages collected from bronchoalveolar lavage fluid

Serum concentration of atrial natriuretic peptide (ANP) (72h)72 hours after study inclusion

ANP is secreted primarily by atrial cardiomyocytes

Sequential organ failure assessment (SOFA) score72 hours after study inclusion

0-43, higher scores correspond to more severe sepsis

Mechanical ventilation time after inclusionUp to 8 weeks

Patients requiring mechanical ventilation after study inclusion

90-day readmission rateThrough study completion, an average of 2 year

Percentage of readmission to hospital within 90 days of study inclusion

CCL1 expression in alveolar macrophages (72 h)72 hours after study inclusion

CCL1 expression in alveolar macrophages collected from bronchoalveolar lavage fluid

expression of WNT pathway proteins in alveolar macrophages (0 h)0 hour after study inclusion

Wnt5a, FZD5, b-catenin, Lef1 expression in alveolar macrophages collected from bronchoalveolar lavage fluid

expression of WNT pathway proteins in alveolar macrophages (72 h)72 hours after study inclusion

Wnt5a, FZD5, b-catenin, Lef1 expression in alveolar macrophages collected from bronchoalveolar lavage fluid

Trial Locations

Locations (1)

Department of Critical Care Medicine, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology

🇨🇳

Wuhan, Hubei, China

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