Healing Effect of Ziziphus Honey on Extracted Tooth Socket in Humans by Evaluating the Levels of BMP-2 and OPN

Phase 3

Completed

• Conditions: Healing Wound
• Interventions: Drug: ziziphus honey

• Registration Number: NCT05025306
• Lead Sponsor: Postgraduate Medical Institute, Lahore

Brief Summary

Ziziphus honey can improve bone healing at extracted tooth sockets in humans by increasing salivary levels of bone markers, OPN and BMP-2.

Detailed Description

objectives of study: To study the effects of ziziphus honey on bone healing of extracted tooth socket in humans by comparing 1) Levels of BMP-2 and OPN in experimental and control groups at different intervals and 2) By radiographically measuring RBD of extraction sockets in experimental and control groups.

Aim of study: Post extraction ridge resorption is a major concern for future tooth prosthesis or implant placement. Honey can possibly help to improve bone healing and minimize ridge resorption after extractions.

Materials and Methods: Randomized controlled trial with simple random sampling of healthy individuals will be done. Total number of 30 participants will be equally divided into experimental group and control group. After tooth extractions in both groups, ziziphus honey will be injected into extracted sockets of experimental group while control group will be left as it is. Levels of BMP-2 and OPN will be tested in saliva through ELISA technique at day 0, 3 and 7 of tooth extraction and RBD will be measured on periapical radiographs at day 3, 21 and 40 in both groups.

Keywords: Bone morphogenetic protein-2 (BMP-2), Osteopontin (OPN), Relative bone density (RBD), Enzyme linked immunosorbent assay (ELISA).

Study Timeline

• Study Start: 2020-01-01
• Primary Completion: 2020-03-16
• Study Completion: 2020-06-01
• Status Verified: 2021-08
• Study First Submitted: 2021-08-23
• Study First Submitted QC: 2021-08-23
• Last Update Submitted: 2021-08-26
• Study First Posted: 2021-08-27
• Last Update Posted: 2021-09-01

Recruitment & Eligibility

• Status: COMPLETED
• Sex: All
• Target Recruitment: 30

Inclusion Criteria

1. Healthy patients of either gender with adult age
2. No known hypersensitivity to honey.
3. Permanent maxillary or mandibular molars (first or second molars)

Exclusion Criteria

1. History of radiotherapy or chemotherapy
2. Diabetic and hypertensive patients.
3. Pregnant and lactating females.
4. Smoking or consumption of alcohol
5. Bone conditions like osteoporosis etc.
6. Patients on oral contraceptives or steroid therapy
7. Subjects taking antibiotics or NSAIDS within one week.

Study & Design

• Study Type: INTERVENTIONAL
• Study Design: PARALLEL

Arm && Interventions

Group	Intervention	Description
experimental group	ziziphus honey	ziziphus honey was applied into the sockets after tooth extractions in experimental group

Primary Outcome Measures

Name	Time	Method
levels of BMP-2	1 week	bone morphogenetic protein-2 levels to access amount of bone healing
Relative bone density	40 days	bone quality confirmed on periapical radiographs in both groups
levels of OPN	1 week	Osteopontin levels to access amount of bone healing

Trial Locations

Locations (1)

Post graduate medical institute, Lahore; 🇵🇰 Lahore, Punjab, Pakistan