Effects of Disrupting Prolonged Sitting With Different Physical Activity Protocols on Metabolic Risk Factors in Sedentary Adults

Not Applicable

• Conditions: Breaking Prolonged Sitting With Physical Activity; Prolonged Sitting
• Interventions: Behavioral: Breaking prolonged sitting with physical activity

• Registration Number: NCT02215603
• Lead Sponsor: Rigshospitalet, Denmark

Brief Summary

Great controversy exists with respect to the optimal or the minimum volume, intensity, and frequency of physical activity capable of attenuating the hazards of prolonged sitting on the metabolic profile. Thus, our study aims to comprehensively investigate the effects of disrupting prolonged sitting with different physical activity protocols on metabolic risk factors in sedentary males.

Ten sedentary males will take part in this randomized cross-over trial consisting of four 27-h conditions. All conditions will be identical except for the physical activity: prolonged sitting intervention (SIT) participants will sit continuously for 9 hours; prolonged sitting+interval standing intervention (STAND), participants will stand for 15 min every 30 minutes (total 270 min) during the 9 hours of sitting; prolonged sitting+moderate-intensity exercise bout (MVPA) participants will perform a 30-min moderate-intensity exercise bout on a treadmill (energy-matched to STAND), after which they will sit for the remaining time; and prolonged sitting+moderate-intensity exercise bout +interval standing intervention (MVPA-STAND), participants will perform a 30-min moderate-intensity exercise bout on a treadmill (energy-matched to STAND), after which they will stand for 15 min every 30 minutes (total 240 min) during the remaining 8 hours of sitting. Blood glucose, insulin, lipids and cytokines will be determined.

The investigators expect that disrupting prolonged sitting with intermittent standing and a moderate-exercise bout will positively affect the metabolic profile of the participants. Furthermore, we will investigate if combined, these strategies will have an additive effect.

Detailed Description

Not available

Study Timeline

• Study Start: 2014-08
• Study First Submitted: 2014-08-07
• Study First Submitted QC: 2014-08-11
• Study First Posted: 2014-08-13
• Status Verified: 2016-01
• Last Update Submitted: 2016-01-12
• Last Update Posted: 2016-01-13
• Primary Completion: 2016-06
• Study Completion: 2016-12

Recruitment & Eligibility

• Status: UNKNOWN
• Sex: Male
• Target Recruitment: 10

Inclusion Criteria

• Males,
• age 25-55 y,
• BMI >18 and <35 kg/m2

Exclusion Criteria

• Clinically diagnosed diabetes,
• dyslipidaemia,
• hypertension,
• use of glucose- and/or lipid-lowering medication,
• smoking,
• evidence of thyroid, liver, lung, heart or kidney disease,
• non-sedentary occupation and primary means of commuting to work (i.e., cycling) in the last 4 months,
• VO2max levels above the considered average fitness according to age,
• contraindication to increased levels of physical activity.

Study & Design

• Study Type: INTERVENTIONAL
• Study Design: CROSSOVER

Arm && Interventions

Group	Intervention	Description
Moderate exercise bout + Prolonged sitting +Standing bouts	Breaking prolonged sitting with physical activity	30-min moderate-intensity exercise bout and stand bout for 15-min every 30 minutes during the remaining 8 hours of sitting
Moderate exercise bout + Prolonged sitting	Breaking prolonged sitting with physical activity	30-min moderate-intensity exercise bout followed by 8 h of sitting
Prolonged sitting + interval standing bouts	Breaking prolonged sitting with physical activity	Stand bout for 15-min every 30 minutes during the 9 hours of sitting

Primary Outcome Measures

Name	Time	Method
Area under the curve of glucose and insulin postprandial responses	27 h	Glucose and insulin levels will be assessed 10 min before, and 30, 60, 120 and 180 min after each main meal. These postprandial responses will be defined as the area under the curve (AUC) assessed over the 3-h period after each main meal (3-h AUC) and the cumulative AUC after all main meals (12-h AUC).

Secondary Outcome Measures

Name	Time	Method
Glucose levels	27 hours	Glucose incursions will measured using the glucose monitoring (CGM) system throughout the 27 hours. Mean, maximum and minimum glucose values during the 27h will be the outcome measures (mmol/l).
Area under the curve of lipid postprandial responses	27 h	Lipid levels will be assessed 10 min before, and 30, 60, 120 and 180 min after each main meal. These postprandial responses will be defined as the area under the curve (AUC) assessed over the 3-h period after each main meal (3-h AUC) and the cumulative AUC after all main meals (12-h AUC).

Trial Locations

Locations (1)

Centre of Inflammation and Metabolism; 🇩🇰 Copenhagen, Denmark