Mechanisms for Laser-Induced Rejuvenation
试验速览
- 阶段
- 不适用
- 状态
- 招募中
- 入组人数
- 12
- 试验地点
- 2
- 主要终点
- To test if possible cells transitioning from monocytes to fibroblasts are found in the top part of the dermis (papillary dermis).
研究概览
简要总结
It has shown that laser wounding of geriatric skin protects it against skin cancer. The purpose of this study is to determine the origin of cells called fibroblasts which are present after treatment with Fractionated Laser Resurfacing (FLR). These cells restore a youth-like ultraviolet B (UVB) response, but where they come from is currently unknown. The study recruits subjects who are scheduled to undergo abdominoplasties to investigate the origin of those cells stimulated by laser treatment.
研究设计
- 研究类型
- Interventional
- 分配方式
- Na
- 干预模型
- Single Group
- 主要目的
- Prevention
- 盲法
- None
入排标准
- 年龄范围
- 18 Years 至 —（Adult, Older Adult）
- 性别
- All
- 接受健康志愿者
- 否
入选标准
- •Adult males and females 18 and older
- •Able to comprehend procedures and risks
- •Planning to undergo abdominoplasty surgery
- •Minimum amount of skin to be removed is 50 x 20cm
排除标准
- •Medical history of not well-controlled diabetes. This is defined as a subject who needs high doses of insulin, recent history of hospitalization for diabetes-related complications, or poor wound health as evidenced by diabetic ulcers. Subjects who have been deemed healthy enough for abdominoplasty surgery would tend to suggest that their diabetes should not be an issue. However, if the subjects have diabetic complications as outlined above, they could affect the wound healing which is the object of this protocol so they would be excluded.
- •Pregnant or nursing
- •History of pathologically abnormal wound healing such as keloidal scars
- •Existing tattoos on the abdomen that are so extensive that they could interfere with the study
研究组 & 干预措施
Fractionated Laser Resurfacing
At 14 days prior to scheduled surgery a 5x5cm square on the left abdomen within the region of skin to be excised and treated with fractionated laser resurfacing. Then at 7 days prior to scheduled surgery a 5 cm diameter circle on the right abdomen within the region of skin to be excised, at least 10 cm away from the square, is treated with fractionated laser resurfacing.
干预措施: Fractionated Laser Resurfacing (Device)
结局指标
主要结局
To test if possible cells transitioning from monocytes to fibroblasts are found in the top part of the dermis (papillary dermis).
时间窗: 2 weeks prior to skin removal and 1 week prior to skin removal
Using tissue RNA-seq, PI will define if co-expressing cells are in the papillary dermis.
To test if the new fibroblasts that develop in response to fractionated laser resurfacing (FLR) wounding are derived from blood-derived monocytes.
时间窗: 2 weeks prior to skin removal and 1 week prior to skin removal
Identify cells in the skin that have been wounded by FLR at 2 weeks and 1 week previously, for cells that co-express markers for both fibroblasts and monocytes by using single cell RNA-sequencing
次要结局
- To define the locations of the types of cells populating in the skin following fractionated laser resurfacing (FLR) wounding(2 weeks prior to skin removal and 1 week prior to skin removal)
- To define the number and types of cells populating the skin following fractionated laser resurfacing (FLR) wounding(2 weeks prior to skin removal and 1 week prior to skin removal)
- To define the locations of the number of cells populating in the skin following fractionated laser resurfacing (FLR) wounding(2 weeks prior to skin removal and 1 week prior to skin removal)