Oxidative DNA Damage Enhancement by Vitamin C Among Narghile Smokers and Non-smokers
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 发起方
- 入组人数
- 96
- 试验地点
- 1
- 主要终点
- Oxidative DNA damage
研究概览
简要总结
This study aims to evaluate oxidative DNA damage among hookah smokers compared to non-smokers by measuring biomarkers of oxidative stress in blood samples. The objective is to determine the impact of hookah smoking on DNA integrity and overall oxidative status in adults.
详细描述
Hookah smoking has become increasingly popular in many regions, yet its potential impact on oxidative stress and DNA integrity is not fully understood. This study aims to investigate the level of oxidative DNA damage among hookah smokers compared to non-smokers by analyzing biomarkers in blood samples. A case-control observational design will be used, involving two groups of adult participants: regular hookah smokers and age-matched non-smokers. Blood samples will be collected from all participants and analyzed for markers of oxidative stress, including 8-hydroxy-2'-deoxyguanosine (8-OHdG) and malondialdehyde (MDA), using validated biochemical methods. The findings are expected to provide insights into the biological consequences of hookah smoking and support the development of public health interventions.
研究设计
- 研究类型
- Interventional
- 分配方式
- Randomized
- 干预模型
- Parallel
- 主要目的
- Basic Science
- 盲法
- Single (Participant)
入排标准
- 年龄范围
- 18 Months 至 30 Months（Child）
- 性别
- All
- 接受健康志愿者
- 否
入选标准
- •Adults aged 18-30 years
- •Current narghile (hookah) smokers for at least 1 year
- •Willing to provide informed consent
排除标准
- •Healthy participant
研究组 & 干预措施
Narghile smokers
this group take vitamin c supplent for one month period and are healthy.
干预措施: Vitamin C (Ascorbic Acid) regularly has been taken (Dietary Supplement)
Narghile nonsmokers vitamin C taking
this group of participant take vitamin C for one month, and they are healthy volunteers
干预措施: Vitamin C (Ascorbic Acid) regularly has been taken (Dietary Supplement)
结局指标
主要结局
Oxidative DNA damage
时间窗: Baseline and after 1 month
Measurement of plasma MDA concentration using the thiobarbituric acid reactive substances (TBARS) method to assess oxidative stress in participants before and after 1-month Vitamin C supplementation and measuring oxidative stress marker and TAOC
次要结局
未报告次要终点
研究者
Nora Tariq Mohammed
MSc Student in Biochemistry
University of Duhok