Pilot Study of Haploidentical Natural Killer Cell Infusions for Poor Prognosis Non-AML Hematologic Malignancies
- Conditions
- Acute Lymphoblastic LeukemiaChronic Myelogenous LeukemiaJuvenile Myelomonocytic LeukemiaMyelodysplastic SyndromeNon-Hodgkin's Lymphoma
- Interventions
- Other: NK Cell InfusionBiological: ImmunotherapyDevice: Miltenyi Biotec CliniMACS device
- Registration Number
- NCT00697671
- Lead Sponsor
- St. Jude Children's Research Hospital
- Brief Summary
The prognosis of pediatric patients with hematologic malignancies whose disease is primarily refractory or those who experience a chemotherapy resistant bone marrow relapse is extremely poor. When new agents or chemotherapeutic regimens are unable to induce remission in this patient population, hematopoietic stem cell transplant (HSCT) is also a poor alternative. Thus, in this very high risk group, additional attempts at remission induction with various combinations of chemotherapy alone will unlikely improve outcome and will contribute to overall toxicity. Alternative therapies are needed in these patients with chemotherapy resistant disease.
Immunotherapy with natural killer (NK) cell infusion has the potential to decrease toxicity and induce hematologic remission. NK cells can kill target cells, including leukemia cells, without prior exposure to those cells. In patients undergoing allogeneic HSCT, several studies have demonstrated the powerful effect of NK cells against leukemia. Furthermore, NK cell infusions in patients with primary refractory or multiple-relapsed leukemia have been shown to be well tolerated and void of graft-versus-host disease effects. In this high risk group, complete leukemic remission has been observed in several of these patients after NK cell infusion.
With the current technology available at St. Jude, we have developed a procedure to purify NK cells from adult donors. This protocol will assess the safety of chemotherapy and IL-2 administration to facilitate transient NK-cell engraftment in research participants who have chemotherapy refractory hematologic malignancies including acute lymphoblastic leukemia, chronic myelogenous leukemia, juvenile myelomonocytic leukemia, myelodysplastic syndrome, or non-Hodgkin's lymphoma. In this same cohort, we will also intend to explore the efficacy of NK cells infused in those participants who have chemotherapy refractory disease.
- Detailed Description
This study will evaluate the persistence, phenotype and function of donor NK cells as well as exploring the efficacy of the infusion in research participants with chemotherapy refractory hematologic malignancies.
Recruitment & Eligibility
- Status
- COMPLETED
- Sex
- All
- Target Recruitment
- 48
- At least two weeks since receipt of last biological therapy, chemotherapy, or radiation therapy.
- Has a suitable adult family member donor available for NK cell donation.
- No current pleural or pericardial effusion.
- HIV negative
- Adequate clinical standing as evidenced by being within multiple renal, hepatic, pulmonary, and neurological required testing parameters.
- Pregnant or lactating
Study & Design
- Study Type
- INTERVENTIONAL
- Study Design
- PARALLEL
- Arm && Interventions
Group Intervention Description Strata A NK Cell Infusion Patients with ALL, CML, JMML, MDS, or NHL with bone marrow relapse after stem cell transplant. Strata A Miltenyi Biotec CliniMACS device Patients with ALL, CML, JMML, MDS, or NHL with bone marrow relapse after stem cell transplant. Strata A Interleukin-2 (IL-2) Patients with ALL, CML, JMML, MDS, or NHL with bone marrow relapse after stem cell transplant. Strata A Immunotherapy Patients with ALL, CML, JMML, MDS, or NHL with bone marrow relapse after stem cell transplant. Strata B NK Cell Infusion Patients with ALL, CML, JMML , MDS, or NHL with primary induction failure and persistent disease; or participants with relapsed ALL, CML, JMML, MDS, or NHL with persistent disease after re-induction Strata B Immunotherapy Patients with ALL, CML, JMML , MDS, or NHL with primary induction failure and persistent disease; or participants with relapsed ALL, CML, JMML, MDS, or NHL with persistent disease after re-induction Strata B Miltenyi Biotec CliniMACS device Patients with ALL, CML, JMML , MDS, or NHL with primary induction failure and persistent disease; or participants with relapsed ALL, CML, JMML, MDS, or NHL with persistent disease after re-induction Strata B Interleukin-2 (IL-2) Patients with ALL, CML, JMML , MDS, or NHL with primary induction failure and persistent disease; or participants with relapsed ALL, CML, JMML, MDS, or NHL with persistent disease after re-induction Strata B Etoposide Patients with ALL, CML, JMML , MDS, or NHL with primary induction failure and persistent disease; or participants with relapsed ALL, CML, JMML, MDS, or NHL with persistent disease after re-induction Strata A Etoposide Patients with ALL, CML, JMML, MDS, or NHL with bone marrow relapse after stem cell transplant. Strata A Clofarabine Patients with ALL, CML, JMML, MDS, or NHL with bone marrow relapse after stem cell transplant. Strata A Cyclophosphamide Patients with ALL, CML, JMML, MDS, or NHL with bone marrow relapse after stem cell transplant. Strata B Clofarabine Patients with ALL, CML, JMML , MDS, or NHL with primary induction failure and persistent disease; or participants with relapsed ALL, CML, JMML, MDS, or NHL with persistent disease after re-induction Strata B Cyclophosphamide Patients with ALL, CML, JMML , MDS, or NHL with primary induction failure and persistent disease; or participants with relapsed ALL, CML, JMML, MDS, or NHL with persistent disease after re-induction
- Primary Outcome Measures
Name Time Method To assess the safety of chemotherapy and IL-2 administration to facilitate transient NK-cell engraftment in research participants with chemotherapy refractory non-acute myelogenous leukemia (non-AML) hematologic malignancies 4 months post infusion
- Secondary Outcome Measures
Name Time Method To study the persistence, phenotype and function of donor natural killer (NK) cells after infusion in research participants with chemotherapy refractory hematologic malignancies. 4 months infusion To explore the efficacy of NK cell infusion in research participants with chemotherapy refractory hematologic malignancies 4 months infusion
Trial Locations
- Locations (1)
St. Jude Children's Research Hospital
🇺🇸Memphis, Tennessee, United States