Nutritional Strategies to Augment the Postprandial Muscle Protein Synthetic Response to the Ingestion of a Low Dose of Protein in Middle-aged Women
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 50
- 试验地点
- 2
- 主要终点
- The myofibrillar protein synthetic response to protein ingestion
研究概览
简要总结
Using stable isotope methodology, investigators will determine the postabsorptive and postprandial muscle protein synthetic response in 60 female adults (Age 50-79, BMI: <30 kg/m2) immediately after an acute bout of resistance exercise. Participants will be divided in to 5 groups of twelve women (n=12). Each group will receive a different protein beverage or placebo to consume following the resistance exercise training. Group 1 will consume a beverage consisting of whey protein; group 2 will consume whey protein supplemented with additional leucine; group 3 will consume whey protein supplemented with additional leucine and hydrolyzed whey peptides; group 4 will consume whey protein supplemented with additional leucine and citrulline; group 5 will just consume water. During the testing, blood and muscle samples will be collected.
研究设计
- 研究类型
- Interventional
- 分配方式
- Randomized
- 干预模型
- Parallel
- 主要目的
- Basic Science
- 盲法
- Single (Participant)
入排标准
- 年龄范围
- 20 Years 至 79 Years（Adult, Older Adult）
- 性别
- Female
- 接受健康志愿者
- 是
入选标准
- •BMI <30 kg/m2
- •Healthy, sedentary
- •Either 20-30 years old or 50-79 years old
排除标准
- •Tobacco use
- •Allergies to milk consumption
- •Unusually high protein consumption
- •Vegan diet
- •Allergies to antibiotics or xylocaine
- •Phenylketonuria (PKU)
- •Diagnosed GI tract diseases
- •Arthritic conditions
- •A history of neuromuscular problems
- •Heart disease
- •Metabolic disorders
- •-Liver, kidney, or urinary disease
- •Musculoskeletal problems
- •Autoimmune disease
- •Neurological disease
- •Previous participation in amino acid tracer studies
- •Predisposition to hypertrophic scarring or keloid formation
- •Individuals on any medications known to affect protein metabolism (i.e. corticosteroids, non-steroidal anti-inflammatories, or prescription strength acne medications).
- •Individuals using thyroid medications or other medications affecting endocrine function
- •Pregnancy
- •Contraindications for exercise
- •supplements that influence protein metabolism (e.g omega 3 fish oils)
结局指标
主要结局
The myofibrillar protein synthetic response to protein ingestion
时间窗: Postabsorptive for 3 hours, Postprandial for 4 hours hours
Myofibrillar protein synthesis rates will be assessed during the postabsorptive period for 3 hr and during the 4 hr after the ingestion of the experimental beverages. This will allow us to assess the change from the postabsorptive to the postprandial period
次要结局
- mTORC1 phosphorylation(Baseline and at 2 and 4 hours after protein ingestion)
研究者
Nicholas Burd
Assistant Professor
University of Illinois at Urbana-Champaign