Effect of Green tea on mutans streptococci

Phase 3

Completed

Brief Summary: The present research is a randomized, parallel arm, controlled trial designed to compare the effect of 2% Green tea extract with 0.2% Chlorhexidine mouthrinse on the Mutans Streptococci count in the saliva and salivary pH. The results could be summarised as:

1. There was a statistically significant reduction in the Mutans Streptococci colonies in the saliva after 0.2% chlorhexidine mouthrinse.

2. There was a statistically significant reduction in the Mutans Streptococci colonies in the saliva after 2% Green tea extract mouthrinse.

3. Significant difference was observed between the Mutans Streptococcus Count (CFU/ml) between the 0.2% Chlorhexidine group and 2% Green tea group.

4. There is a rise in the salivary pH towards neutrality after rinsing with the 0.2% Chlorhexidine mouthrinse but this is not statistically significant.

5. The inhibition of the fall in the salivary pH after 2% Green tea extract mouthrinse is clearly demonstrated and is statistically significant.

Thus we can conclude that, 2% Green tea was considerably efficacious in reducing the Mutans Streptococci count

significantly, although not superior to the gold standard 0.2% Chlorhexidine. It proves to be a promising anticariogenic mouthrinse with its acid inhibition property in this research by maintaining the salivary pH near neutrality significantly, even after a sucrose challenge and can be used as a cost effective and safe caries inhibitory agent.

Recruitment & Eligibility

Inclusion Criteria

Exclusion Criteria

1.Those Patients who received antibiotic therapy at last 3 weeks, 2.Those who had topical fluoride application at last 48 hours, 3.Those who had topical mouthwash application at last 48 hours and 4.Those who ate or drank within last 2 hours before the study 5.Those with limited manual dexterity.
6.Those with systemic illness/mental illness/physically handicapped 7.Those giving a history of allergy to any of the above constituents of mouthrinses.
8.Those who are pregnant 9.Those who are uncooperative.

Primary Outcome Measures

Name	Time	Method
mutans streptococci CFU/ml and pH of saliva taken in all the salivary samples	A salivary sample (sample A) was obtained from the subjects after which a 10% sucrose solution was given. After 7 minutes, Post rinse samples (sample B) were collected in After a waiting period of one hour, a pre rinse saliva (sample C). Following this, 2% Green tea or 0.2% Chlorhexidine rinse was given. After a 20 minute interval, a 10% sucrose solution was given for 2 minutes. After 7 minutes, Post rinse samples (sample B) were collected in the same manner.

Secondary Outcome Measures

Name	Time	Method

Locations (1): Senthamil college
🇮🇳
Madurai, TAMIL NADU, India
Senthamil college
🇮🇳Madurai, TAMIL NADU, India
Dr Priscilla JoysN
Principal investigator
9003677047
mailpriscy18@gmail.com