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The Impact of Maternal Microbes on Infant Health Programming

Completed
Conditions
Healthy
Registration Number
NCT03552939
Lead Sponsor
Institute of Agrochemistry and Food Technology, National Research Council
Brief Summary

MAMI aims to characterize maternal microbes to be transferred to neonates and determine their function in infant health programming.

Detailed Description

Recent reports suggest that early microbial colonization has an important role for in promoting health. This may contribute to reduce the risk of chronic diseases such as obesity, allergies and inflammatory conditions. Advances in understanding host-microbe interactions imply that maternal microbiota plays a crucial role on health programming. This process begins in utero and it is modulated by mode of delivery and diet. The investigator's previous data has shown that i) specific shifts in milk microbial composition are associated with lactation time and mode of delivery, ii) milk microbes drive the infant microbiota composition; iii) maternal microbiota dysbiosis may be transferred to the infant. However, factors defining maternal microbiota and its biological role upon infant's health are not yet fully understood. Hence, this project aims to characterize maternal microbes to be transferred to neonates and determine their function in infant health programming. The specific aims are:(1) understanding how the maternal microbiome is influenced by host and environmental factors;(2) characterizing the microbial core and bioactive compounds transmitted to the offspring mainly via breastfeeding and their key roles in the microbial modulation and host response;(3) understanding the interactions among breast milk bioactive compounds and their role in infant health;(4) shedding light on how maternal microbes influence the infant immune system.

Results obtained will demonstrate the interaction between infant nutrition, microbes and host response in early life and its key role in health programming, enabling new applications in the field of personalized nutrition \& medicine.

Recruitment & Eligibility

Status
COMPLETED
Sex
All
Target Recruitment
250
Inclusion Criteria
  • Age> 18 years.
  • Healthy woman (no medication, no diabetes, no pre-gestational thyroid problems)
  • Postpartum woman (beginning of the puerperium).

Exclusion criteria:

  • Non-compliance with any of the inclusion criteria.
  • Medication and drugs
  • Health problems at the immunological and metabolic levels.
Exclusion Criteria

Not provided

Study & Design

Study Type
OBSERVATIONAL
Study Design
Not specified
Primary Outcome Measures
NameTimeMethod
Microbiota compositionfrom birth to 24 month

Dominant microbial species maternal and infant samples (using qPCR and sequencing -approaches analysis

Secondary Outcome Measures
NameTimeMethod
Maternal dietfrom birth to12 month

Maternal diet (FFQ) during gestation and 12 month post-partum

Maternal weightpre-gestational and from birth to 12 months post-partum

To check Weight gain over pregnancy (kg) and weight during lactation

Maternal BMIpre-gestational

To check Body Mass Index (kg/cm2)

To identify the impact of mode of deliveryBirth

Mode of delivery type: Vaginal/C-section (elective or non-elective)

To identify maternal factors affecting microbiota: antibioticspre-gestational

antibiotics treatment during gestation (number of treatments)

Infant weightfrom birth to 24 month

Infant weight (kg)

Infant heightfrom birth to 24 month

Infant height (cm)

Infant Dietfrom bith to 24 month

Exclusive breastfeeding time (months), breastfeeding duration and time of introduction of complementary food

Fecal short-chain fatty acidsfrom birth to 24 month

Determine the relationship between fecal microbiota composition and fecal short chain fatty acids

Trial Locations

Locations (2)

Hospital Universitario y Politecnico la Fe

🇪🇸

Valencia, Spain

Fundacion Investigacion Sanitaria INCLIVA

🇪🇸

Valencia, Spain

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