Changes in the Distribution of Mitochondria During Oocyte Maturation
- Conditions
- Infertility
- Interventions
- Other: Fixation and stainingOther: Observation by laser microscopy
- Registration Number
- NCT01501292
- Lead Sponsor
- Hillel Yaffe Medical Center
- Brief Summary
Cytoplasmatic maturation is an important event in the general maturation of of the oocyte. Mitochondria is one of the major organelles involved in the process of cytoplasmatic maturation. The aim of this study is to investigate the presence and distribution of the mitochondria in the cytoplasma of immature oocytes that are retrieved in the routine in-vitro maturation cycle with intracytoplasmatic sperm injection (ICSI). These oocytes will not undergo ICSI because of their immaturity. Usually, they are discarded. In addition to these immature oocytes the investigators shall also investigate the mitochondria in mature oocytes that underwent ICSI but did not become fertilized and are discarded.
- Detailed Description
Not available
Recruitment & Eligibility
- Status
- UNKNOWN
- Sex
- Female
- Target Recruitment
- 20
- Infertile patients needing IVF treatment
- Patients not requiring the ICSI procedure
Study & Design
- Study Type
- OBSERVATIONAL
- Study Design
- Not specified
- Arm && Interventions
Group Intervention Description Immature oocytes (GV, M-I) Fixation and staining - Immature oocytes (GV, M-I) Observation by laser microscopy - Mature oocytes (M-II) Fixation and staining - Mature oocytes (M-II) Observation by laser microscopy -
- Primary Outcome Measures
Name Time Method The patterns of mitochondrial distribution within the cytoplasma of oocytes during nuclear maturation Three months Identification the different patterns of mitochondrial distribution in correlation with the 3 stages nuclear maturation in human oocytes
- Secondary Outcome Measures
Name Time Method
Trial Locations
- Locations (1)
Hillel Yaffe Medical Center
🇮🇱Hadera, Israel