Acute Effect of Fructose on Lipid Metabolism and Gender Differences

Not Applicable

Completed

• Conditions: Lipid Metabolism
• Interventions: Dietary Supplement: Fructose

• Registration Number: NCT00620360
• Lead Sponsor: University of Lausanne

Brief Summary

It has been widely documented that fructose overfeeding increases plasma triglycerides and hepatic de novo lipogenesis, and impairs insulin sensitivity in healthy male volunteers. The effect of gender on the metabolic responses to fructose remains an important open question, however.

The objective of this study is to compare the effect of an acute oral fructose load on carbohydrate and lipid metabolism in healthy young males and females.

Detailed Description

The study is aimed at comparing the effects of oral fructose on several specific metabolic pathways in males and females.Participants will receive an isoenergetic diet containing 55% carbohydrate, 15% protein and 35% lipids for three days prior to testing. After this period of controlled diet, they will be studied for 2 hours in the post-absorptive state (Time 0-120 min) and over a 6 hours period (Time 120-480 min) during which they will receive 4 loads of 0,30 g/kg fat free mass U-13C labelled fructose, at times 120, 180, 240, 300. Throughout the study, deuterated glucose and glycerol will be infused to monitor whole body glucose production and glycerol turnover.

The following parameters will be monitored in basal conditions and after the ingestion of the load of fructose:

* Glycerol turnover(glycerol 2H5)

* de novo lipogenesis (incorporation of 13C into palmitate of VLDLs)

* whole body energy expenditure and net substrate oxydation (indirect calorimetry)

* net fructose oxidation (breath 13CO2)

* glucose turnover: (6,6 2H2 Glucose)

* plasma glucose, free fatty acids, ketone bodies, lactate, insulin, triacylglycerol, total cholesterol, VLDL, LDL, and HDL subfractions

An adipose tissue (periumbilical subcutaneous) biopsy will be obtaine by needle aspiration under local anesthesia in fasting conditions (time 0 min) and after fructose (time 480 min) to assess the effects of fructose on adipose gene expression profile. Key genes involved in the regulation of carbohydrate (GLUT 4, hexokinase, PDH-kinase), lipid (FAT-CD36, FABP, acetylCoA carboxylase, malonyl-CoA decarboxylase, PPARg) and energy metabolism (PGC-1a, UCP2)will be monitored

Results obtained in males and females will be compared with two-way analysis of variance

Study Timeline

• Study Start: 2008-01
• Study First Submitted: 2008-02-08
• Study First Submitted QC: 2008-02-08
• Study First Posted: 2008-02-21
• Primary Completion: 2008-12
• Study Completion: 2008-12
• Status Verified: 2012-02
• Last Update Submitted: 2012-02-23
• Last Update Posted: 2012-02-24

Recruitment & Eligibility

• Status: COMPLETED
• Sex: All
• Target Recruitment: 18

Inclusion Criteria

• Males and females
• Age 18-35
• Healthy
• Body mass indexes (BMI) between 19 and 25 kg/m2
• Informed consent obtained

Exclusion Criteria

• Smokers
• Alcohol intake > 30g/day
• Drug abuse
• Diabetes mellitus

Study & Design

• Study Type: INTERVENTIONAL
• Study Design: SINGLE_GROUP

Arm && Interventions

Group	Intervention	Description
fructose	Fructose	acute fructose administration

Primary Outcome Measures

Name	Time	Method
Hepatic de novo lipogenesis	acute effect of dietary fructose (within 6 hours)

Secondary Outcome Measures

Name	Time	Method
Whole body lipid oxidation, glucose turnover, glycerol turnover, plasma substrates, hormone and energy expenditure (free fatty acid, glucose, lactate, beta-hydroxybutyrate, glycerol, VLDL- Triglycerides and insulin) expression of key adipose genes	acute effect of fructose (within 6 hours)

Trial Locations

Locations (1)

Centre Hospitalier Universitaire Vaudois; 🇨🇭 Lausanne, Vaud, Switzerland