The Influence of 16:8 Time-Restricted Eating on Resistance Exercise-Induced Skeletal Muscle Hypertrophy
Overview
- Phase
- Not Applicable
- Intervention
- Not specified
- Conditions
- Muscle Hypertrophy
- Sponsor
- California State University, Fullerton
- Enrollment
- 20
- Locations
- 1
- Primary Endpoint
- Changes in Muscle Size - Lower Body
- Status
- Completed
- Last Updated
- 3 years ago
Overview
Brief Summary
The purpose is to examine the effects of time-restricted eating (TRE; consuming all calories within an 8-hour period each day) vs. normal eating (CON; consuming same kcals and protein as TRE, but during a 10-13 hr eating window each day) during 8 weeks of resistance exercise on body composition and muscle mass (whole muscle and single fiber), muscular performance, anabolic protein signaling, single muscle fiber characteristics, and the gut microbiome in well-trained young men and women.
Detailed Description
The primary goal pertains to muscle hypertrophy (not fat loss), thus all participants will be placed in a 10% caloric surplus.
Investigators
Andrew Galpin
Associate Professor
California State University, Fullerton
Eligibility Criteria
Inclusion Criteria
- •18-35 years old with \<25% (men) or \<30% (women) body fat
- •have experience in resistance exercise for the whole body and performed resistance exercise at least 3 times per week the last 12 months
- •be able to perform all necessary exercises with safe technique and no pain
- •be able to squat 1.5x (men) or 1x (women) body weight
- •be able to bench press 1.1x (men) or 0.55x (women) body weight
- •be on a non-specialized, mixed macronutrient diet
- •have a lifestyle which enables a reasonably consistent 7+ hours of sleep per night
- •be free of any current joint, musculoskeletal, and/or neuromuscular injuries
Exclusion Criteria
- •have any known current illness, prohibitive muscular or neuromuscular problem, hypertension, cardiac, pulmonary, liver, kidney, insulin or any other metabolic disorders (e.g. Type I or Type II diabetes)
- •are lactose intolerant or unable to consume whey protein for any reason
- •are on any medications known to affect protein metabolism
- •have taken antibiotics in the last 6 months
- •cannot refrain from consuming any alcoholic beverages, nicotine, or marijuana (or derivative) for a period of 48 hours prior to data collection (pre and post testing)
- •consume alcohol \>2x per week
- •consume any analgesic or anti-inflammatory drug(s), prescription or non-prescription, chronically or within 48 hours of data collection
- •consume any recreational drug(s), prescription or non-prescription, legal or illegal, chronically or within 30 days of data collection
- •consume any caffeine or food within 12 hours of data collection
- •cannot refrain from physical exercise (outside of activities of daily living) outside of what is prescribed by the research team for the duration of the study
Outcomes
Primary Outcomes
Changes in Muscle Size - Lower Body
Time Frame: Pre, Mid (4 weeks), and Post (8 weeks)
via Ultrasound
Changes in Muscle Size - Upper Body
Time Frame: Pre, Mid (4 weeks), and Post (8 weeks)
via Ultrasound
Changes in Fiber Type-Specific Size
Time Frame: Pre and Post (week 8)
Single muscle fiber analysis
Changes in Body composition
Time Frame: Pre, Mid (4 weeks), and Post (8 weeks)
via BodPod
Acute Exercise Induced Fiber Type-Specific Anabolic Signaling (Total Concentration and Phosphorylation Status)
Time Frame: Day 1 of exercise training
Men only; assessed via single fiber Simple Western
Secondary Outcomes
- Changes in Maximal Strength(Pre and Post (week 8))
- Changes in Maximal Muscular Endurance(Pre and Post (week 8))
- Changes in Enjoyment of Diet(Weekly for duration of the intervention (8 weeks))
- Changes in Sleep (total and quality)(Weekly for duration of the intervention (8 weeks))
- Changes in Gut Microbiome(Pre, Mid (week 2, 4, 6), and Post (week 8))
- Changes in Fiber Type Composition(Pre and Post (week 8))
- Changes in Fiber Type-Specific Resting Anabolic Signaling (Total Concentration and Phosphorylation Status)(Pre and Post (week 8))
- Changes in Menstrual Cycle(Pre, Mid (week 4), and post (week 8))
- Total Training Volume(After 8 weeks)
- Nutrient Intake(Daily, starting 7 days before the study and continuing for 8 additional weeks)
- Changes in Mood(Weekly for duration of the intervention (8 weeks))