Quantification of Organophosphorus and Paraquat compound using gas chromatography-mass spectrometry in Acute Poisoning Patients and its Correlation with Clinical Severity Scores and Patient Outcomes : A prospective observational study in a tertiary care emergency setting.
试验速览
- 阶段
- 不适用
- 状态
- 尚未招募
- 入组人数
- 75
- 试验地点
- 1
- 主要终点
- 1. Quantification of organophosphorus compound using GC-MS
研究概览
简要总结
Title : Quantification of Organophosphorus(Chlorpyrifos) and paraquat compounds using Gas chromatography-mass spectrometry in acute poisoning patients and its correlation with clinical severity and patient outcomes.
Background: Acute pesticide poisoning is a leading cause of morbidity and mortality in India, particularly chlorpyrifos and paraquat herbicide. Clinical tools are widely used but have significant limitations as they rely on markers or clinical estimation rather than direct toxin burden. This study aims to evaluate whether GC-MS based quantification of OP and Paraquat levels correlate better with severity and clinical outcomes.
Study Summary: This is a prospective observational study to be conducted at Kasturba Medical Hospital, Manipal over a period of 2 years and 4 months. A total of 50 Chlorpyrifos OP poisoning and 25 paraquat poisoning will be enrolled, with using blood samples collected within six hours of admission with a informed consent in the patient’s own language. The collected sample will be transported to the biochemical lab under cold chain and stored in -80C. Quantified levels using GC-MS will be correlated with standard severity scores, biochemical markers and patient outcomes including 28 day mortality and morbidity will be recorded using hospital records and telephonic follow-up.
研究设计
- 研究类型
- Observational
入排标准
- 年龄范围
- 18.00 Year(s) 至 85.00 Year(s)（—）
- 性别
- All
入选标准
- •Adults above 18 years All patients with Clinical diagnosis of acute Organophosphorus (Chlorpyrifos) or Paraquat poisoning Presentation within 48 hours post-exposure.
排除标准
- •Mixed or multiple substance ingestion.
结局指标
主要结局
1. Quantification of organophosphorus compound using GC-MS
时间窗: 1. At admission | 2. At admission | 3. At 28 days
2. Clinical severity in OP poison patients (POP score)
时间窗: 1. At admission | 2. At admission | 3. At 28 days
3. Clinical severity scoring for Paraquat poison (SIPP,serum paraquat, APACHE
时间窗: 1. At admission | 2. At admission | 3. At 28 days
And Mortality
时间窗: 1. At admission | 2. At admission | 3. At 28 days
次要结局
- 1. Mortality in Organophosphorus poisoning(2. Morbidity indicator (vasopressor-free days, fewer ventilator-free days, or prolonged ICU stay) in paraquat poisoning patients)
研究者
Chaitanya CH
Kasturba Medical College, Manipal