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Intravenous Methylene Blue for Treating Refractory Neonatal Septic Shock

Phase 2
Not yet recruiting
Conditions
Neonatal Sepsis
Shock, Septic
Interventions
Other: Placebo
Registration Number
NCT06306001
Lead Sponsor
Post Graduate Institute of Medical Education and Research, Chandigarh
Brief Summary

Preterm infants (born at less than 37 weeks of pregnancy) sometimes develop a serious blood infection leading to low blood pressure, which does not respond to saline or to the standard medicines for increasing blood pressure, such as dopamine and epinephrine. The goal of this research study is to compare the effect of giving an injectable medicine called Methylene blue (MB) versus not giving MB to such preterm infants who are unresponsive to standard treatment. The main questions that this study aims to answer is:

1. Whether MB treatment reduces death to any cause as compared to no MB treatment.

2. Whether treatment with MB reduces the time to achieve normal blood pressure

3. Whether treatment with MB reduces the time to stoppage of all blood pressure medications, steroids and normal saline.

4. Whether treatment with MB improves heart function as measured by echocardiography at 24 and 48 hours.

Detailed Description

Preterm infants with definite or probable sepsis and fluid-refractory, catecholamine-resistant septic shock will be eligible for enrolment if they have no contraindication to receive MB. After obtaining parental consent, they will be stratified as per the first-line catecholamine used and randomly allocated to receive MB (bolus followed by infusion) or no MB for 24 hours. They will be observed for all-cause mortality (primary outcome), cause-specific mortality, time to achieve hemodynamic stability and adverse effects (secondary outcomes) over a 7-day period, all-cause mortality and cause-specific mortality hospital stay and duration of hospital stay.

The main questions it aims to answer are

1. To determine whether treatment with intravenous MB therapy reduces all-cause mortality when compared to no MB treatment, among preterm neonates with catecholamine-resistant septic shock

2. To compare the time to achieve therapeutic endpoints among preterm neonates with catecholamine-resistant septic shock treated with intravenous MB versus no MB

3. To compare time to stoppage of all inotrope/vasopressor treatment among preterm neonates with catecholamine-resistant septic shock treated with intravenous MB versus no MB

4. To compare echocardiographic parameters (at 24 hours after randomization) among preterm neonates with catecholamine-resistant septic shock treated with intravenous MB versus no MB

Recruitment & Eligibility

Status
NOT_YET_RECRUITING
Sex
All
Target Recruitment
130
Inclusion Criteria

Not provided

Exclusion Criteria

excluded if ≥1 criterion positive:

  1. G6PD deficient or family history of G6PD deficiency
  2. Potentially lethal malformation
  3. Congenital heart disease
  4. Severe acute kidney injury
  5. Family history of allergy to methylene blue or food dyes

Study & Design

Study Type
INTERVENTIONAL
Study Design
PARALLEL
Arm && Interventions
GroupInterventionDescription
Placebo infusionPlaceboSubjects in the control arm will receive a placebo infusion (normal saline) at the same volumetric rate.
Methylene blueMethylene BlueSubjects in the intervention arm will receive a 1 mg/kg bolus of methylene blue over 30 minutes, followed by an infusion of 0.15 mg/kg/h. The infusion rate may be increased in steps of 0.15 mg/kg/h every 30 minutes until a maximum of 0.5 mg/kg/h.
Primary Outcome Measures
NameTimeMethod
All-cause mortality within 7 days after randomization7 days

Mortality due to any cause over 7 days after randomization

Secondary Outcome Measures
NameTimeMethod
Time taken to achieve therapeutic end-points within 7 days after randomization7 days

Time taken to achieve therapeutic end points of shock (which include capillary refill time less than 3 seconds, normal volume pulses, warm extremities, urine output greater than 1 ml/kg/h, normal sensorium, normal mean blood pressure, normal systolic blood pressure and normal diastolic blood pressure) up to 7 days after randomization.

Time taken to stop all inotrope/vasopressor treatment within 7 days after randomisation7 days

Time taken for all inotrope and vasopressor therapy to finally stop up to a maximum of 7 days after randomisation

Echocardiographic fractional shortening at 24 hour after randomization24 hour

Fractional shortening will be calculated on echocardiography by measuring the percentage change in the left ventricular diameter during systole at 24 hours after randomization.

Septic shock-related mortality7 days

Mortality attributed to septic shock up to 7 days post-randomisation

Left ventricular end-systolic diameter (LVESD) by echocardiography at 24 hour after randomization24 hour

Left ventricular end-systolic diameter (LVESD) will be measured in millimeters by echocardiography at 24 hour after randomization

Aortic diameter by echocardiography at 24 hour after randomization24 hour

Aortic diameter will be measured in millimeters by echocardiography at 24 hour after randomization

Mortality during hospital stay100 days

Mortality during the period of hospital stay up to a maximum of 100 days

Left ventricular end-diastolic diameter (LVEDD) by echocardiography at 24 hour after randomization24 hour

Left ventricular end-diastolic diameter (LVEDD) will be measured in millimetres by echocardiography at 24 hour after randomization

Velocity time integral (LVI) by echocardiography at 24 hours after randomization24 hour

Velocity time integral (LVI) will be measured in centimeters by echocardiography at 24 hours after randomization to calculate the cardiac output.

Echocardiographic fractional shortening at 48 hour after randomization48 hour

Fractional shortening will be calculated on echocardiography by measuring the percentage change in the left ventricular diameter during systole at 48 hours after randomization.

Left ventricular end-diastolic diameter (LVEDD) on echocardiography at 48 hour after randomization48 hour

Left ventricular end-diastolic diameter (LVEDD) will be measured in millimeters by echocardiography at 48 hour after randomization

Aortic diameter by echocardiography at 48 hour after randomization48 hour

Aortic diameter will be measured in millimeters by echocardiography at 48 hour after randomization

Velocity time integral (LVI) by echocardiography at 48 hours after randomization48 hour

Velocity time integral (LVI) be measured by echocardiography in centimeters at 48 hours after randomization to calculate the cardiac output.

Time taken to stop vasopressor treatment100 days

Time taken to stop all vasopressors during hospital stay up to a maximum of 100 days

Left ventricular end-systolic diameter (LVESD) by echocardiography at 48 hour after randomization48 hour

Left ventricular end-systolic diameter (LVESD) will be measured in millimeters by echocardiography at 48 hour after randomization

Serious adverse effects100 days

Serious adverse effect with special reference to oliguria, gastrointestinal bleeds, abdominal distension, and bluish discoloration of skin and urine during hospital stay up to a maximum of 100 days

Trial Locations

Locations (1)

Post Graduate Institute of Medical Education and Research (PGIMER)

🇮🇳

Chandigarh, India

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