Skin Fibroblasts for Cholesterol Research

Completed

• Conditions: Cholesterol metabolism; lipid disorder; 10011082; 10003216; 10013317

• Registration Number: NL-OMON41130
• Lead Sponsor: Academisch Medisch Centrum

Brief Summary

Not available

Detailed Description

Not available

Study Timeline

• Last Update Posted: 23 April 2024

Recruitment & Eligibility

• Status: Completed
• Sex: Not specified
• Target Recruitment: 100

Inclusion Criteria

- Patients with extreme tail LDL-C, HDL-C or TG levels, defined as >95% or <5% adjusted for sex and age, with a putative or proven molecular defect.
- Patients with premature atherosclerosis, defined as a first cardiovascular event <50 years for men and <55 years for women.
- Healthy family members as control
- Participating in Biobank Vascular Medicine (METC2014_082) and gave written consent for re-contact by the investigators for follow-up studies.

Exclusion Criteria

Age <18 years

Study & Design

• Study Type: Observational invasive
• Study Design: Not specified

Primary Outcome Measures

Name	Time	Method
<p>In human skin fibroblasts we study:<br /><br>- Cholesterol uptake. (LDL binding and uptake assays)<br /><br>- Receptor trafficking for receptors involved in cholesterol metabolism<br /><br>(LDL-receptor, ABCA1 and ABCG1).<br /><br>- Cholesterol efflux from the cells. </p><br>

Secondary Outcome Measures

Name	Time	Method
<p>not applicable</p><br>