Développement d'un Atlas Des Interactions hôte-microbiome Respiratoire Chez Des Volontaires Sains
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 82
- 试验地点
- 1
- 主要终点
- Atlas of host-microbiome interactions
研究概览
简要总结
The role of the pulmonary microbiome in the pathophysiology of lung disease is becoming increasingly well understood. Studies on murine models have highlighted the immunomodulatory and antibacterial activity of certain commensal bacteria, such as S. mitis, and have led to an understanding of their potential therapeutic impact. The investigators will therefore couple their analyses of the respiratory microbiome with an in-depth analysis of mucosal immunity. In particular, they will characterize immune cell types and frequencies using high-dimensional (spectral) flow cytometry and single cell RNA-sequencing. Finally, they will study the mediators exchanged between the microbiome and the host using proteomic and metabolomic analyses of respiratory fluids.
研究设计
- 研究类型
- Interventional
- 分配方式
- Na
- 干预模型
- Single Group
- 主要目的
- Other
- 盲法
- None
入排标准
- 年龄范围
- 18 Years 至 70 Years（Adult, Older Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •Male or female.
- •Age from 18 to
- •Indication for scheduled general anesthesia with orotracheal intubation for non-thoracic, non-carcinological, non-septic surgical procedures such as prosthesis (knee or hip), spinal surgery, digestive hernia surgery, gall bladder surgery, maxillofacial surgery (non-exhaustive list).
- •Membership of a social security scheme or beneficiary of such a scheme.
- •Written informed consent.
排除标准
- •Persons under guardianship or trusteeship.
- •Pregnant or breast-feeding woman.
- •Antibiotic treatment received within 28 days prior to surgery, regardless of the site of infection.
- •Acute respiratory pathology within the last 28 days (pneumothorax, bronchitis or pneumonia including Covid).
- •History of chronic respiratory disease (asthma, chronic obstructive pulmonary disease, emphysema, lung cancer, cystic fibrosis, etc.).
- •Immunosuppression (cancer active or within the last 5 years, HIV, radio-chemotherapy within the last year, organ transplant).
- •Chronic inflammatory disease (digestive, osteoarticular, neurological or cutaneous) requiring or having required specific treatment in the last 12 months.
- •Type I or II diabetes.
- •Steroid treatment within the last 30 days (regardless of dose or duration).
研究组 & 干预措施
Voluntary
Subjects undergoing scheduled surgery in the operating room at Nantes University Hospital, requiring general anesthesia with placement of an endotracheal intubation tube for invasive mechanical ventilation.
干预措施: Voluntary (Other)
结局指标
主要结局
Atlas of host-microbiome interactions
时间窗: 1 day
Healthy respiratory metacluster
次要结局
- Correlations between bacterial species(Day 1)
- Correlations between cell populations(Day 1)
- Correlations between metabolite concentrations(Day 1)
- Correlations between protein concentrations(Day 1)
- Correlations between host-microbiome interactions and quality of life scales(Day 1)