Interest of High-speed Centrifugation for Measuring Anti-Xa Activity on Lipemic Plasma

Recruiting

• Conditions: Hypertriglyceridemia

• Registration Number: NCT07200674
• Lead Sponsor: University Hospital, Strasbourg, France

Brief Summary

Some hemostasis techniques require spectrophotometric measurements. These are then subject to interference from the sample's lipemia (postprandial, hypertriglyceridemia, etc.), which risks distorting the results; this is estimated to affect 0.5 to 2.5% of samples.

This is particularly the case for anti-Xa activity, a critical test used to monitor anticoagulant treatments.

To date, the HUS Hematology Laboratory does not have or is aware of any alternative method to overcome this interference, and is unable to perform anti-Xa activity when the sample has excessive lipemia. This study will evaluate, on samples from routine care artificially overloaded with lipids, the effectiveness of a high-speed centrifugation process (10,800g for 10 minutes).

Detailed Description

Not available

Study Timeline

• Study Start: 2024-03-11
• Status Verified: 2025-03
• Study First Submitted: 2025-03-26
• Study First Submitted QC: 2025-09-22
• Last Update Submitted: 2025-09-22
• Study First Posted: 2025-10-01
• Last Update Posted: 2025-10-01
• Primary Completion: 2025-12-11
• Study Completion: 2025-12-11

Recruitment & Eligibility

• Status: RECRUITING
• Sex: All
• Target Recruitment: 150

Inclusion Criteria

• Adult patient (≥ 18 years)
• Patient anticoagulated with an anti-Xa (UFH, LMWH, fondaparinux, danaparoid, apixaban, rivaroxaban)
• Sample sent to the HUS Hematology Laboratory and analyzed for measurement of the anti-Xa activity of an anticoagulant as part of routine care

Exclusion Criteria

• Patient with a basal lipidemic sample
• Sample of insufficient volume

Study & Design

• Study Type: OBSERVATIONAL
• Study Design: Not specified

Primary Outcome Measures

Name	Time	Method
To determine whether high-speed centrifugation (10,800g for 10 minutes) effectively separates the lipid fraction from the rest of the plasma	Up to 10 minutes	To determine whether high-speed centrifugation (10,800g for 10 minutes) effectively separates the lipid fraction from the rest of the plasma, in order to measure anti-Xa activity, and to ensure that this process does not interfere with this measurement.

Trial Locations

Locations (1)

Laboratoire d'Hématologie - Unité d'Hémostase - CHU de Strasbourg - France; 🇫🇷 Strasbourg, France